F-Luc Self-Amplifying RNA (Cap1, m5C)

The Firefly-Luciferase (F-Luc) self-amplifying RNA (saRNA) expresses firefly luciferase protein, which is the enzyme for bioluminescence of fireflies and click beetles. After efficient delivery into cells or animals, F-Luc mRNA can translate into F-Luc and emit bioluminescence in the presence of its substrate, Luciferin. This saRNA is capped with Cap1-AU structure with high capping efficiency. It has 100% substituted with 5-methylcytidine for enhanced expression and reduced immunogenicity. The saRNA has a ~70A tail in its sequence, mimics a mature mRNA.