| Species |
Human |
| Protein Construction |
Expressed with an N-terminal Met.
PDGF-BB (Ser82-Thr190)
Accession # P01127 |
|
| Purity |
> 97% as analyzed by SDS-PAGE
> 97% as analyzed by HPLC |
| Endotoxin Level |
< 1 EU/μg of protein by LAL method |
| Biological Activity |
Fully biologically active when compared to standard. The ED50 as determined by a cell proliferation assay using murine Balb/c 3T3 cells is less than 3.0 ng/ml, corresponding to a specific activity of > 3.3 × 105 IU/mg. |
| Expression System |
E. coli |
| Theoretical Molecular Weight |
24.8 kDa |
| Formulation |
Lyophilized from a 0.2 μm filtered solution in PBS, pH 7.4. |
| Reconstitution |
It is recommended that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute the lyophilized powder in sterile distilled water or aqueous buffer containing 0.1 % BSA to a concentration of 0.1-1.0 mg/ml. |
| Storage & Stability |
Upon receiving, this product remains stable for up to 6 months at -70°C or -20°C. Upon reconstitution, the product should be stable for up to 1 week at 4°C or up to 3 months at -20°C. Avoid repeated freeze-thaw cycles. |
Lane 1: 2 μg of PDGF-BB, Human, non-reducing (NR)
Lane 2: 2 μg of PDGF-BB, Human, reducing (R)
> 97% as analyzed by SDS-PAGE
| Target Background |
Platelet-Derived Growth Factor-BB (PDGF-BB) is one of five dimers (PDGF-AA, AB, BB, CC, and DD) formed by 4 different PDGF subunits. In vivo, PDGF-BB is mainly produced in heart and placenta, and predominantly expressed by osteoblasts, fibroblasts, smooth muscle cells, and glial cells. An inactive precursor of PDGF-BB is produced in the endoplasmic reticulum and then activated by a proprotein convertase after secretion. PDGF-BB functions in a paracrine manner and promotes organogenesis, human skeletal development, and wound healing. PDGF-BB also promotes angiogenesis, particularly in the presence of Fibroblast Growth Factor basic. Therefore, PDGF-BB and its related pathways are potential pharmacological targets. |
| Synonyms |
PDGFBB; IBGC5; PDGF-2; PDGF2; SIS; SSV; c-sis; platelet derived growth factor subunit B |
For laboratory research use only. Direct human use, including taking orally and injection and clinical use are forbidden.
