2 μg of IFN-γ, Human (Cat. No. Z02915) was resolved with SDS-PAGE under reducing (R) and non-reducing (N) conditions and visualized by Coomassie Blue staining.

Biological Activity
IFN-gamma, Human (Cat. No. Z02915) induced cytotoxicity of HT-29 (HTB-38) cells. The ED₅₀ for this effect is less than 0.05ng/mL.

IFN-γ, Human

Human Interferon gamma (hIFN-γ) is amacrophage‐activating factor and the lone member of Interferon type II.The active form of IFN-γ is an antiparallel dimer that interacts with the receptor IFN-γR1 and sets off IFN-γ/JAK/STAT pathway. IFN-γ signaling does diverse biological functions primarily related to host defense and immune regulation, including antiviral and antibacterial defense, apoptosis, inflammation, and innate and acquired immunity. While IFN-γ–induced inflammatory cascade summons a variety of immune‐related cell types, such as macrophages, natural killer (NK) cells and cytotoxic T lymphocytes (CTLs), IFN-γ is also implicated in resistance to NK cell and CTL responses and in immune escape in a variety of cancers.

产品编号	Z02915
规格	10 μg 50 μg 100 μg 1 mg 大单询价
数量
价格	￥200


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产品简介

Species

Human

Protein Construction

Expressed with an N-terminal Met.

IFN-γ (Gln24-Gln166)
Accession # P01579

Purity

> 95% as analyzed by SDS-PAGE

Endotoxin Level

< 1 EU/μg of protein by gel clotting method

Biological Activity

ED₅₀ < 0.05 ng/ml, measured by cytotoxicity assay using HT‐29 cells.

Expression System

E. coli

Apparent Molecular Weight

~17 kDa, on SDS-PAGE under reducing conditions.

Formulation

Lyophilized after extensive dialysis against PBS.

Reconstitution

It is recommended that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute the lyophilized powder in ddH₂O or PBS up to 100 μg/ml.

Storage & Stability

Upon receiving, this product remains stable for up to 6 months at lower than -70°C. Upon reconstitution, the product should be stable for up to 1 week at 4°C or up to 3 months at -20°C. For long term storage it is recommended that a carrier protein (example 0.1% BSA) be added. Avoid repeated freeze-thaw cycles.

图例

2 μg of IFN-γ, Human (Cat. No. Z02915) was resolved with SDS-PAGE under reducing (R) and non-reducing (N) conditions and visualized by Coomassie Blue staining.

Biological Activity
IFN-gamma, Human (Cat. No. Z02915) induced cytotoxicity of HT-29 (HTB-38) cells. The ED₅₀ for this effect is less than 0.05ng/mL.

技术背景

Target Background

Human Interferon gamma (hIFN-γ) is amacrophage‐activating factor and the lone member of Interferon type II.The active form of IFN-γ is an antiparallel dimer that interacts with the receptor IFN-γR1 and sets off IFN-γ/JAK/STAT pathway. IFN-γ signaling does diverse biological functions primarily related to host defense and immune regulation, including antiviral and antibacterial defense, apoptosis, inflammation, and innate and acquired immunity. While IFN-γ–induced inflammatory cascade summons a variety of immune‐related cell types, such as macrophages, natural killer (NK) cells and cytotoxic T lymphocytes (CTLs), IFN-γ is also implicated in resistance to NK cell and CTL responses and in immune escape in a variety of cancers.

Synonyms

Immune Interferon; type II interferon; T cell interferon; MAF; IFNG; IFG; IFI; IFN-gamma

For laboratory research use only. Direct human use, including taking orally and injection and clinical use are forbidden.

IFN-γ, Human

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