Biological Activity
IL-11, Human (Cat. No. Z03108) stimulates cell proliferation of T11 cells. The ED₅₀ for this effect is less than 5ng/mL.

ED₅₀ < 5.0 ng/ml, measured in a proliferation assay using TF-1 cells.

Lane 1: 2 μg of IL-11, Human(CHO-expressed), reducing (R)
Lane 2: 2 μg of IL-11, Human(CHO-expressed), non-reducing (NR)
> 95% as analyzed by SDS-PAGE

IL-11, Human(CHO-expressed)

Interleukin-11 is a pleiotropic cytokine that was originally detected in the conditioned medium of an IL-1α-stimulated primate bone marrow stromal cell line (PU-34) as a mitogen for the IL-6-responsive mouse plasmacytoma cell line T11. IL-11 has multiple effects on both hematopoietic and non-hematopoietic cells. Many of the biological effects described for IL-11 overlap with those for IL-6. In vitro, IL-11 can synergize with IL-3, IL-4 and SCF to shorten the G0 period of early hematopoietic progenitors. IL-11 also enhances the IL-3-dependent megakaryocyte colony formation. IL-11 has been found to stimulate the T cell-dependent development of specific immunoglobulin-secreting B cells.

产品编号	Z03108
规格	5 μg 25 μg 1 mg 大单询价
数量
价格	￥600


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产品简介

Species

Human

Protein Construction

IL-11 (Pro22-Leu199)
Accession # P20809-1

Purity

> 95% as analyzed by SDS-PAGE
> 95% as analyzed by HPLC

Endotoxin Level

< 0.2 EU/μg of protein by gel clotting method

Biological Activity

ED₅₀ < 5.0 ng/ml, measured in a proliferation assay using TF-1 cells.

Expression System

CHO

Apparent Molecular Weight

~23 kDa, on SDS-PAGE under reducing conditions.

Formulation

Lyophilized after extensive dialysis against PBS.

Reconstitution

It is recommended that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute the lyophilized powder in ddH₂O or PBS up to 100 μg/ml.

Storage & Stability

Upon receiving, this product remains stable for up to 6 months at lower than -70°C. Upon reconstitution, the product should be stable for up to 1 week at 4°C or up to 3 months at -20°C. For long term storage it is recommended that a carrier protein (example 0.1% BSA) be added. Avoid repeated freeze-thaw cycles.

图例

Biological Activity
IL-11, Human (Cat. No. Z03108) stimulates cell proliferation of T11 cells. The ED₅₀ for this effect is less than 5ng/mL.

ED₅₀ < 5.0 ng/ml, measured in a proliferation assay using TF-1 cells.

Lane 1: 2 μg of IL-11, Human(CHO-expressed), reducing (R)
Lane 2: 2 μg of IL-11, Human(CHO-expressed), non-reducing (NR)
> 95% as analyzed by SDS-PAGE

技术背景

Target Background

Interleukin-11 is a pleiotropic cytokine that was originally detected in the conditioned medium of an IL-1α-stimulated primate bone marrow stromal cell line (PU-34) as a mitogen for the IL-6-responsive mouse plasmacytoma cell line T11. IL-11 has multiple effects on both hematopoietic and non-hematopoietic cells. Many of the biological effects described for IL-11 overlap with those for IL-6. In vitro, IL-11 can synergize with IL-3, IL-4 and SCF to shorten the G0 period of early hematopoietic progenitors. IL-11 also enhances the IL-3-dependent megakaryocyte colony formation. IL-11 has been found to stimulate the T cell-dependent development of specific immunoglobulin-secreting B cells.

Synonyms

IL11; AGIF; IL-11; interleukin 11

For laboratory research use only. Direct human use, including taking orally and injection and clinical use are forbidden.

IL-11, Human(CHO-expressed)

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