| Species |
Mouse |
| Protein Construction |
Expressed with an N-terminal Met.
CXCL9 (Thr22-Thr126)
Accession # P18340 |
|
| Purity |
> 95% as analyzed by SDS-PAGE |
| Endotoxin Level |
< 0.2 EU/μg of protein by gel clotting method |
| Biological Activity |
The EC50 value of Mouse MIG/CXCL9 on Ca2+ mobilization assay in CHO-K1/Gα15/mCXCR3 cells (human Gα15 and mouse CXCR3 stably expressed in CHO-K1 cells) is less than 2.0 μg/ml. |
| Expression System |
E. coli |
| Apparent Molecular Weight |
~12.3 kDa, on SDS-PAGE under reducing conditions. |
| Formulation |
Lyophilized after extensive dialysis against PBS. |
| Reconstitution |
It is recommended that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute the lyophilized powder in ddH₂O or PBS up to 100 μg/ml. |
| Storage & Stability |
Upon receiving, this product remains stable for up to 6 months at lower than -70°C. Upon reconstitution, the product should be stable for up to 1 week at 4°C or up to 3 months at -20°C. For long term storage it is recommended that a carrier protein (example 0.1% BSA) be added. Avoid repeated freeze-thaw cycles. |
| Target Background |
Chemokine (C-X-C motif) ligand 9 (CXCL9), also known as monokine induced by interferon gamma (MIG), is a small cytokine belonging to the CXC chemokine family.The CXCL9 gene is induced in macrophages and in primary glialcells of the central nervous systemin response to IFNγ. CXCL9 has been shown to be achemo attractant for activated Th1lymphocytes and tumor-infiltrating leukocytes (TILs) but not for neutrophils or monocytes. CXCL is also involved in other cellular activities including inhibition of tumor growth, angiogenesis, and inhibition of colony formation of hematopoietic progenitors. CXCL9 is closely related to two other CXC chemokines, CXCL10 and CXCL11.CXCL9, CXCL10 and CXCL11 all elicit their chemotactic functions by interacting with the chemokine receptor CXCR3. |
| Synonyms |
CXCL-9; CMK; Humig; MIG; SCYB9; crg-10; C-X-C motif chemokine ligand 9; Small inducible cytokine B9; Gamma interferon-induced monokine; monokine induced by gamma-interferon |
For laboratory research use only. Direct human use, including taking orally and injection and clinical use are forbidden.
