GenCrispr NLS-Cas9-EGFP Nuclease
¥915 | |
Z03393 | |
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|
现货 | |
|
|
|
¥915 | |
Z03393 | |
|
|
现货 | |
|
|
|
|
1000 nM is equal to 190 ng/µl.
Storage & Stability | GenCrispr NLS-Cas9-EGFP is supplied with 1X storage buffer (10 mM Tris, 300 mM NaCl, 0.1 mM EDTA, 1 mM DTT, 50% Glycerol pH7.4 at 25 °C) and recommended to be stored at -20 °C. |
In vitro DNA cleavage assay with GenCrispr NLS-Cas9-EGFP nuclease
Reactions were set up according to recommended conditions, and cleavage products were resolved on a 1% agarose gel. Input DNA is EcoR V-linearized pUC57 plasmid DNA. Lane 1, DNA + gRNA; lane 2, marker; lane 3 and 4, DNA + gRNA + NLS-Cas9-EGFP 100 ng (lane 3) or 50 ng (lane 4). »
Left: Cell transfection assay. 12 h after electroporation, cells were observed under bright or fluorescence microscope.
Right: in vivo gene editing efficiency assay by T7E1. Lane 1, marker; lane 2, negative control; lane 3, gRNA + NLS-Cas9-EGFP; lane 4, gRNA + NLS-Cas9. »
A 20 μl reaction in 1 × Cas9 Nuclease Reaction Buffer containing linearized plasmid, gRNA, and NLS-Cas9-EGFP for 1 hour at 37 °C results in a digestion efficiency of linearized plasmid higher than 90%, as determined by agarose gel electrophoresis. »