| Species |
Human |
| Protein Construction |
EpCAM/Trop1 (Gln24-Lys245)
Accession # P16422 |
Poly-His |
| N-term |
C-term |
|
| Purity |
> 95% as analyzed by SDS-PAGE
> 95% as analyzed by HPLC |
| Endotoxin Level |
≤ 1 EU/μg of protein by LAL method |
| Biological Activity |
Immobilized Human EpCAM at 0.2 μg/ml (100 μl/Well). Dose response curve for Anti-Trop1 Ab with the EC50 of 8.7 ng/ml determined by ELISA. |
| Expression System |
Expi293 |
| Apparent Molecular Weight |
30~45 kDa, on SDS-PAGE under reducing conditions. |
| Formulation |
Lyophilized from a 0.22 μm filtered solution in PBS, pH 7.4. Normally 5 % trehalose is added as protectant before lyophilization. |
| Reconstitution |
It is recommended that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute the lyophilized powder in distilled water up to 100 μg/ml. |
| Storage & Stability |
Upon receiving, this product remains stable for up to 6 months at -70°C or -20°C. Avoid repeated freeze-thaw cycles. |
Immobilized Human EpCAM at 0.2 μg/ml(100 μl/Well). Dose response curve for Anti-Trop1 Ab with the EC50 of 8.7 ng/ml determined by ELISA.
Recombinant EpCAM on SDS-PAGE under reducing conditions, the purity is greater than 95%.
The purity of EpCAM is greater than 95% as determined by SEC-HPLC.
| Target Background |
Epithelial Cellular Adhesion Molecule (EpCAM), also known as KS1/4, gp40, GA733-2, 17-1A, and TROP‑1, is a 40 kDa transmembrane glycoprotein that consists of a 242 amino acid (aa) extracellular domain with two EGF‑like repeats, a 23 aa transmembrane segment, and a 26 aa cytoplasmic domain. |
| Synonyms |
CD326 antigen; CD326; EGP; EGP-2; EGP314; EGP40; EpCAM; epithelial cell adhesion molecule; ACSTD1; antigen identified by monoclonal AUA1; Cell surface glycoprotein Trop-1; DIAR5; Epithelial glycoprotein 314; Epithelial glycoprotein; gp40; HNPCC8; KS 1/4 antigen; KS1/4; KSAHEA125; M1S2; M4S1; M4S1Ly74; Major gastrointestinal tumor-associated protein GA733-2; MOC31; TACST-1; TACSTD1; TROP1; Tumor-associated calcium signal transducer 1CO-17A |
For laboratory research use only. Direct human use, including taking orally and injection and clinical use are forbidden.
