| Species |
Bovine |
| Protein Construction |
| His tag |
Transferrin (Asp20-Pro704)
Accession # Q29443 |
His tag |
| N-term |
|
C-term |
|
| Purity |
≥ 90% as analyzed by SDS-PAGE |
| Endotoxin Level |
< 0.2 EU/μg of protein by gel clotting method |
| Biological Activity |
ED50 < 5.0 μg/mL, determined by a dose-response proliferation assay using human K562 cells. |
| Expression System |
Fungi |
| Theoretical Molecular Weight |
76.7 kDa |
| N-terminal Sequence Analysis |
His tag |
| Apparent Molecular Weight |
~ 76 kDa, on SDS-PAGE under reducing conditions. |
| Formulation |
Lyophilized from a 0.2 μm filtered solution in 20 mM PBS, pH7.4 |
| Reconstitution |
Before opening, centrifuge the vial briefly to bring the contents to the bottom. Reconstitute the lyophilized powder in ddH2O up to 1 mg/ml. |
| Storage & Stability |
Upon receiving, this product remains stable up to 6 months at -20 °C or below. Upon reconstitution, the product should be stable up to 1 week at 4 °C or up to 3 months at -20 °C. Avoid repeated freeze-thaw cycles. |
Lane 1: 2 µg of Transferrin, Bovine reducing (N)
Lane 2: 2 µg of Transferrin, Bovine reducing (R)
The purity is greater than 90% as analyzed by SDS-PAGE
The ED50 as determined by a cell proliferation assay using human K562 cells is 0.295 μg/ml.
| Target Background |
Transferrin is a blood plasma glycoprotein that plays a central role in iron metabolism and is responsible for ferric-ion delivery. Transferrin functions as the most critical ferric pool in the body. It transports iron through the blood to various tissues, such as the liver, spleen, and bone marrow. |
| Synonyms |
Transferrin |
For research use only. Not intended for human and animal therapeutic or diagnostic use.
