| Species |
Chicken |
| Protein Construction |
EGF (Gly976-Ala1030)
Accession # A0A8V0XEN7 |
|
| Purity |
≥ 95% as analyzed by SDS-PAGE |
| Endotoxin Level |
< 0.2 EU/μg of protein by gel clotting method |
| Biological Activity |
ED50 < 0.2 ng/ml, measured by a cell proliferation assay using BALB/3T3 cells, corresponding to a specific activity of > 5.0 × 106 units/mg. |
| Expression System |
P. pastoris |
| Theoretical Molecular Weight |
6.2 kDa |
| Apparent Molecular Weight |
~ 8.0 kDa, on SDS-PAGE under reducing conditions. |
| Formulation |
Lyophilized from a 0.2 μm filtered solution in 1×PBS, pH 7.2 |
| Reconstitution |
Before opening, centrifuge the vial briefly to bring the contents to the bottom. Reconstitute the lyophilized powder in ddH2O up to 100 μg/ml |
| Storage & Stability |
Upon receiving, this product remains stable up to 6 months at -20 °C or below. Upon reconstitution, the product should be stable up to 1 week at 4 °C or up to 3 months at -20 °C. Avoid repeated freeze-thaw cycles. |
Lane 1: 2 µg of EGF, Chicken under reducing condition (R)
Lane 2: 2 µg of EGF, Chicken under non-reducing condition (NR) (N)
The purity is greater than 95% as analyzed by SDS-PAGE
The ED50 as determined by a cell proliferation assay using BALB/3T3 cells is 0.03128 ng/ml.
| Target Background |
EGF stimulates the growth of various epidermal and epithelial tissues in vivo and in vitro and of some fibroblasts in cell culture. Magnesiotropic hormone that stimulates magnesium reabsorption in the renal distal convoluted tubule via engagement of EGFR and activation of the magnesium channel TRPM6. |
| Synonyms |
Urogastrone; URG; epidermal growth factor |
For research use only. Not intended for human and animal therapeutic or diagnostic use.
