| Species |
Human |
| Protein Construction |
Activin A (Gly311-Ser426)
Accession # P08476 |
|
| Purity |
> 95% as determined by Bis-Tris PAGE |
| Endotoxin Level |
Less than 0.1EU per μg by the LAL method. |
| Biological Activity |
Immobilized Activin A, Human (Cat.No.: Z03814) at 1 μg/ml can bind Human Activin RIIB, His Tag. Activin A, Human (Cat.No.: Z03814) can inhibit proliferation of MPC-11 cells. |
| Expression System |
HEK293 |
| Theoretical Molecular Weight |
12.97 kDa |
| Apparent Molecular Weight |
Due to glycosylation, the protein migrates to 14-15 kDa under reduced (R) condition, and 23-25 kDa under Non reducing (N) condition based on Bis-Tris PAGE result. |
| Formulation |
Lyophilized from 0.22 μm filtered solution in 4 mM HCI. |
| Reconstitution |
Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in 4mM HCI. |
| Storage & Stability |
Upon receiving, the lyophilized product remains stable up to 6 months at -20 °C or below as supplied from date of receipt.-80°C for 3 months after reconstitution.Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles. |
Immobilized Activin A, Human at 1 μg/ml (100 μl/well) on the plate. Dose response curve for Human Activin RIIB, His Tag with the EC50 of 18.6 ng/ml determined by ELISA.
Immobilized Activin A, Human at 1 μg/ml (100 μl/well) on the plate. Dose response curve for Human Activin RIIA, hFc Tag with the EC50 of 2.1 ng/ml determined by ELISA.
Immobilized Activin A, Human on Bis-Tris PAGE under reduced (R) condition and Non reducing (N) condition. The purity is greater than 95%.
Biotinylated Human Activin RIIB, His Tag captured on CM5 Chip via Anti-His Antibody can bind Activin A, Human, No Tag with an affinity constant of 55.35 pM as determined in SPR assay (Biacore T200).
Measured by its ability to inhibit proliferation of MPC-11 cells. The ED50 for this effect is < 7 ng/mL.
| Target Background |
Activin-A, a member of the TGF-β superfamily, has fundamental roles in embryonic development, stem cell maintenance and differentiation, haematopoiesis, cell proliferation, and tissue fibrosis. It signals through two type I and two type II receptors, activating kinase activity, phosphorylating SMAD2 and 3 intracellular signaling mediators, forming a complex with SMAD4, and translocating to the nucleus to regulate gene expression. |
| Synonyms |
INHBA; Activin A; |
For laboratory research use only. Direct human use, including taking orally and injection and clinical use are forbidden.
