Wnt-3a hFc Chimera, Human on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.

Measured by its ability to induce Topflash reporter activity in HEK293T human embryonic kidney cells. The ED50 for this effect is 2-12 ng/mL.

Wnt-3a hFc Chimera, Human

Wnt-3a activates the canonical Wnt signaling pathway and is expressed in the dorsal midline region, playing a crucial role in spinal cord development. Additionally, it regulates autophagy, apoptosis, neuron regeneration, neurogenic inflammation, and axon regeneration. Wnt-3a promotes the beta-catenin/Tcf pathway, which can induce tumors and lead to cancer in specific cell populations. Moreover, it is widely used in cytokine for organoid construction.

产品编号	Z03815
规格	100 μg 500 μg 大单询价
数量
价格	￥3300


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产品简介

Species	Human
Purity	> 95% as determined by Bis-Tris PAGE
Endotoxin Level	Less than 1 EU per μg by the LAL method.
Biological Activity	Wnt-3a hFc Chimera, Human (Cat.No.: Z03815) can induce Topflash reporter activity in HEK293T human embryonic kidney cells.
Expression System	HEK293
Theoretical Molecular Weight	58.50 kDa.
Apparent Molecular Weight	Due to glycosylation, the protein migrates to 60-75 kDa based on Bis-Tris PAGE result.
Formulation	Lyophilized from 0.22 μm filtered solution in PBS, pH 7.4.
Reconstitution	Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water.
Storage & Stability	Upon receiving, the lyophilized product remains stable up to 6 months at -20 °C or below as supplied from date of receipt.-80°C for 3 months after reconstitution.Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

图例

Wnt-3a hFc Chimera, Human on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.

Measured by its ability to induce Topflash reporter activity in HEK293T human embryonic kidney cells. The ED50 for this effect is 2-12 ng/mL.

技术背景

Target Background

Wnt-3a activates the canonical Wnt signaling pathway and is expressed in the dorsal midline region, playing a crucial role in spinal cord development. Additionally, it regulates autophagy, apoptosis, neuron regeneration, neurogenic inflammation, and axon regeneration. Wnt-3a promotes the beta-catenin/Tcf pathway, which can induce tumors and lead to cancer in specific cell populations. Moreover, it is widely used in cytokine for organoid construction.

Synonyms

Wnt-3a; Wnt 3a; WNT3A protein; Wnt3a

For laboratory research use only. Direct human use, including taking orally and injection and clinical use are forbidden.

Wnt-3a hFc Chimera, Human

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