| Species |
Human |
| Protein Construction |
| hFc |
FAP (Leu26-Asp760 )
Accession # Q12884-1 |
| N-term |
C-term |
|
| Purity |
> 95% as determined by Bis-Tris PAGE
> 90% as determined by HPLC |
| Endotoxin Level |
Less than 1EU per μg by the LAL method. |
| Biological Activity |
Immobilized FAP hFc Chimera, Human (Cat.No.: Z03893) at 2 μg/ml (100μl/Well) on the plate can bind Biotinylated Anti-FAP Antibody, hFc Tag |
| Expression System |
HEK293 |
| Theoretical Molecular Weight |
112.3 kDa |
| Apparent Molecular Weight |
Due to glycosylation, the protein migrates to 110-120 kDa based on Bis-Tris PAGE result. |
| Formulation |
Lyophilized from a 0.22 μm filtered solution in PBS, pH 7.4. |
| Reconstitution |
It is recommended that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute the lyophilized powder in ddH2O more than 100 μg/ml. |
| Storage & Stability |
Upon receiving, the product remains stable up to 6 months at -20 °C or below. Upon reconstitution, the product should be stable for 3 months at -80 °C. Avoid repeated freeze-thaw cycles. |
The purity of FAP hFc Chimera, Human is greater than 90% as determined by SEC-HPLC.
FAP hFc Chimera, Human on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
Immobilized FAP hFc Chimera, Human at 2 μg/ml(100 μl/well) on the plate. Dose response curve for Biotinylated Anti-FAP Antibody, hFc Tag with the EC50 of 44.4 ng/ml determined by ELISA.
| Target Background |
Fibroblast activation protein (FAP) is a serine protease that has been reported in fibroblasts and some carcinoma cells, which correlates with poor patient outcomes. FAP can be induced under hypoxia which is also vital in the malignant behaviors of cancer cells. |
| Synonyms |
FAP; FAPalpha; SIMP; Seprase; APCE; DPPIV; DPPIVA; FAPA |
For laboratory research use only. Direct human use, including taking orally and injection and clinical use are forbidden.
