| Species |
Cynomolgus |
| Protein Construction |
| Avi |
His |
FAP (Leu26-Asp760)
Accession # XP_005573377 |
| N-term |
|
C-term |
|
| Conjugate |
Biotin |
| Purity |
> 95% as determined by Bis-Tris PAGE
> 95% as determined by HPLC |
| Endotoxin Level |
Less than 1EU per μg by the LAL method. |
| Biological Activity |
Immobilized FAP[Biotin], His & Avi, Cynomolgus (Cat.No.: Z03896) at 0.5 μg/ml (100μl/Well) on the streptavidin precoated plate can bind Anti-FAP Antibody, hFc Tag |
| Expression System |
HEK293 |
| Theoretical Molecular Weight |
88 kDa |
| Apparent Molecular Weight |
Due to glycosylation, the protein migrates to 90-110 kDa based on Bis-Tris PAGE result. |
| Formulation |
Lyophilized from 0.22 μm filtered solution in 20mM Tris, 500mM NaCl, 200mM L-arginine (pH 8.2). |
| Reconstitution |
It is recommended that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute the lyophilized powder in ddH2O more than 100 μg/ml. |
| Storage & Stability |
Upon receiving, the product remains stable up to 6 months at -20 °C or below. Upon reconstitution, the product should be stable for 3 months at -80 °C. Avoid repeated freeze-thaw cycles. |
![FAP[Biotin], His & Avi, Cynomolgus](/product/documents/down?doc_name=Z03896 ELISA.jpg&file=scm_files/productFile_notes/2025/03/12/Z03896 ELISA.jpg "FAP[Biotin], His & Avi, Cynomolgus")
Immobilized FAP[Biotin], His & Avi, Cynomolgus at 0.5 μg/ml (100 μl/well) on the streptavidin precoated plate(5 μg/ml). Dose response curve for Anti-FAP Antibody, hFc Tag with the EC50 of 6.7 ng/ml determined by ELISA.
![FAP[Biotin], His & Avi, Cynomolgus](/product/documents/down?doc_name=Z03896 PAGE.png&file=scm_files/productFile_notes/2025/03/12/Z03896 PAGE.png "FAP[Biotin], His & Avi, Cynomolgus")
FAP[Biotin], His & Avi, Cynomolgus on Bis-Tris PAGE under reduced conditions. The purity is greater than 95%.
![FAP[Biotin], His & Avi, Cynomolgus](/product/documents/down?doc_name=Z03896 SEC-HPLC.png&file=scm_files/productFile_notes/2025/03/12/Z03896 SEC-HPLC.png "FAP[Biotin], His & Avi, Cynomolgus")
The purity of FAP[Biotin], His & Avi, Cynomolgus is greater than 95% as determined by SEC-HPLC.
| Target Background |
Fibroblast activation protein (FAP) is a serine protease that has been reported in fibroblasts and some carcinoma cells, which correlates with poor patient outcomes. FAP can be induced under hypoxia which is also vital in the malignant behaviors of cancer cells. |
| Synonyms |
FAP; FAPalpha; SIMP; Seprase; APCE; DPPIV; DPPIVA; FAPA |
For laboratory research use only. Direct human use, including taking orally and injection and clinical use are forbidden.
