Immobilized EGFR/HER1[Biotin], His & Avi, Human at 1 μg/ml (100 μl/Well) on streptavidin(5 μg/ml) precoated plate. Dose response curve for Human EGF, hFc Tag with the EC50 of 2.6 ng/ml determined by ELISA.
EGFR/HER1[Biotin], His & Avi, Human on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
The purity of EGFR/HER1[Biotin], His & Avi, Human is greater than 95% as determined by SEC-HPLC.
EGFR/HER1[Biotin], His & Avi, Human captured on CM5 Chip via Anti-his antibody can bind Human EGF, No Tag with an affinity constant of 0.62 nM as determined in SPR assay (Biacore T200).
EGFR/HER1[Biotin], His & Avi, Human
The epidermal growth factor receptor is a transmembrane protein that is a receptor for members of the epidermal growth factor family of extracellular protein ligands. The epidermal growth factor receptor is a member of the ErbB family of receptors, a subfamily of four closely related receptor tyrosine kinases: EGFR, HER2/neu, Her 3 and Her 4. Receptor tyrosine kinase binding ligands of the EGF family and activating several signaling cascades to convert extracellular cues into appropriate cellular responses.
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