Species |
Human |
Protein Construction |
CD2/SRBC (Lys25-Asp209) Accession # P06729 |
His |
Avi |
N-term |
|
C-term |
|
Conjugate |
Biotin |
Purity |
> 95% as determined by Bis-Tris PAGE
> 95% as determined by HPLC |
Endotoxin Level |
Less than 1EU per μg by the LAL method. |
Biological Activity |
Measured by its binding ability in a functional ELISA. Immobilized CD2/SRBC [Biotin], His & Avi, Human at 1μg/ml (100μl/well) on the streptavidin precoated plate (5μg/ml) can bind Human CD58, hFc Tag. Test result was comparable to standard batch. |
Expression System |
HEK293 |
Theoretical Molecular Weight |
24.15 kDa |
Apparent Molecular Weight |
Due to glycosylation, the protein migrates to 35-45 kDa based on Bis-Tris PAGE result. |
Formulation |
Lyophilized from a 0.22 μm filtered solution in PBS,(pH 7.4). |
Reconstitution |
Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water. |
Storage & Stability |
Upon receiving, the product remains stable up to 6 months at -20 °C or below. Upon reconstitution, the product should be stable for 3 months at -80 °C. Avoid repeated freeze-thaw cycles. |
![CD2/SRBC [Biotin], His & Avi, Human CD2/SRBC [Biotin], His & Avi, Human](/product/documents/down?doc_name=Z03984 ELISA.jpg&file=scm_files/productFile_notes/2025/04/25/Z03984 ELISA.jpg)
Immobilized CD2/SRBC [Biotin], His & Avi, Human, His Tag at 1 μg/ml(100 μl/well) on the streptavidin precoated plate(5 μg/ml). Dose response curve for Human CD58, hFc Tag with the EC50 of 1.6 ng/ml determined by ELISA.
![CD2/SRBC [Biotin], His & Avi, Human CD2/SRBC [Biotin], His & Avi, Human](/product/documents/down?doc_name=Z03984 HPLC.png&file=scm_files/productFile_notes/2025/04/25/Z03984 HPLC.png)
The purity of CD2/SRBC [Biotin], His & Avi, Human is greater than 95% as determined by SEC-HPLC.
![CD2/SRBC [Biotin], His & Avi, Human CD2/SRBC [Biotin], His & Avi, Human](/product/documents/down?doc_name=Z03984 PAGE.png&file=scm_files/productFile_notes/2025/04/25/Z03984 PAGE.png)
CD2/SRBC [Biotin], His & Avi, Human on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
Target Background |
The CD2 family of receptors is evolutionarily conserved and widely expressed on cells within the hematopoietic compartment. In recent years several new members have been identified with important roles in the immune system. CD2 family members regulate natural killer (NK) cell lytic activity and inflammatory cytokine production when engaged by ligands on tumor cells. |
Synonyms |
T11; CD2; SRBC; LFA-2; LFA-3 receptor |
For research use only. Not intended for human or animal clinical trials, therapeutic or diagnostic use.