Immobilized CD8 alpha/CD8A[Biotin], His & Avi, Human, His Tag at 0.5 μg/ml (100 μL/well) on the streptavidin precoated plate(5 μg/ml). Dose response curve for Anti-CD8 Antibody, hFc Tag with the EC50 of 4.4 ng/ml determined by ELISA.

CD8 alpha/CD8A[Biotin], His & Avi, Human on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.

CD8 alpha/CD8A[Biotin], His & Avi, Human

CD8 alpha enhances the responses of antigen-specific CTL activated through TCR through binding MHC class I, favoring lipid raft partitioning of TCR, and inducing intracellular signaling. CD8 alpha is also found on dendritic cells and rat macrophages, but whether CD8 alpha enhances responses of a partner receptor, like TCR, to activate these cells is not known.

产品编号	Z04073
规格	100 μg 500 μg 大单询价
数量
价格	￥5800


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产品简介

Species

Human

Protein Construction

CD8 alpha/CD8A (Ser22-Asp182） Accession # P01732-1	His	Avi
N-term		C-term

Conjugate

Biotin

Purity

> 95% as determined by Bis-Tris PAGE

Endotoxin Level

Less than 1EU per μg by the LAL method.

Biological Activity

Measured by its binding ability in a functional ELISA. Immobilized CD8 alpha/CD8A[Biotin], His & Avi, Human at 0.5μg/ml (100μl/well) on the streptavidin precoated plate (5μg/ml) can bind Anti-CD8 Antibody, hFc Tag. Test result was comparable to standard batch.

Expression System

HEK293

Theoretical Molecular Weight

20.5 kDa

Apparent Molecular Weight

Due to glycosylation, the protein migrates to 30-38 kDa based on Bis-Tris PAGE result.

Formulation

Lyophilized from a 0.22 μm filtered solution in PBS, (pH 7.4).

Reconstitution

Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water.

Storage & Stability

Upon receiving, the product remains stable up to 6 months at -20 °C or below. Upon reconstitution, the product should be stable for 3 months at -80 °C. Avoid repeated freeze-thaw cycles.

图例

CD8 alpha/CD8A[Biotin], His & Avi, Human on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.

技术背景

Target Background	CD8 alpha enhances the responses of antigen-specific CTL activated through TCR through binding MHC class I, favoring lipid raft partitioning of TCR, and inducing intracellular signaling. CD8 alpha is also found on dendritic cells and rat macrophages, but whether CD8 alpha enhances responses of a partner receptor, like TCR, to activate these cells is not known.
Synonyms	CD8a; Lyt-2; CD8; Leu2; MAL; p32; CD8a molecule; OKT8 T-cell antigen

For research use only. Not intended for human or animal clinical trials, therapeutic or diagnostic use.