Species |
Cynomolgus |
Protein Construction |
CD30/TNFRSF8 (Ala27-Pro394) Accession # A0A2K5VW07 |
His |
N-term |
C-term |
|
Purity |
> 95% as determined by BisTris PAGE
> 95% as determined by HPLC |
Endotoxin Level |
Less than 1EU per μg by the LAL method. |
Biological Activity |
Measured by its binding ability in a functional ELISA. Immobilized CD30/TNFRSF8, His, Cynomolgus at 2μg/ml (100μl/Well) on the plate can bind AntiCD30 Antibody, hFc Tag. Test result was comparable to standard batch. |
Expression System |
HEK293 |
Theoretical Molecular Weight |
39.86 kDa |
Apparent Molecular Weight |
Due to glycosylation, the protein migrates to 75-105 kDa based on Bis-Tris PAGE result. |
Formulation |
Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). |
Reconstitution |
Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water. |
Storage & Stability |
Upon receiving, the product remains stable up to 6 months at -20 °C or below. Upon reconstitution, the product should be stable for 3 months at -80 °C. Avoid repeated freeze-thaw cycles. |

Immobilized CD30/TNFRSF8, His, Cynomolgus His Tag at 2 μg/ml (100 μl/well) on the plate. Dose response curve for Anti-CD30 Antibody, hFc Tag with the EC50 of 5.1 ng/ml determined by ELISA.

The purity of CD30/TNFRSF8, His, Cynomolgus is greater than 95% as determined by SEC-HPLC.

CD30/TNFRSF8, His, Cynomolgus on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
Target Background |
The transmembrane receptor CD30 (TNFRSF8) and its ligand CD30L (CD153, TNFSF8) are members of the tumor necrosis factor (TNF) superfamily and display restricted expression in subpopulations of activated T-and B-cells in nonpathologic conditions. CD30 expression is upregulated in various hematological malignancies, including Reed-Sternberg cells in Hodgkin's disease (HD), anaplastic large cell lymphoma (ALCL) and subsets of Non-Hodgkin's lymphomas (NHLs). |
Synonyms |
CD30; CD30KI-1; CD30L receptor; TNFRSF8; D1S166EKi-1; CD30KI-1 |
For research use only. Not intended for human or animal clinical trials, therapeutic or diagnostic use.