Immobilized CD27 Ligand/CD70 Trimer, His, Human, His Tag at 2 μg/ml (100 μL/well) on the plate. Dose response curve for Human CD27, hFc Tag with the EC50 of 11.8 ng/ml determined by ELISA.

The purity of CD27 Ligand/CD70 Trimer, His, Human is greater than 95% as determined by SEC-HPLC.

CD27 Ligand/CD70 Trimer, His, Human on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.

CD27 Ligand/CD70 Trimer, His, Human

CD70, also named CD27 ligand (CD27L), is a type II transmembrane glycoprotein belonging to the TNF superfamily (TNFSF) and has been designated TNFSF7. CD70 is a cytokine that binds to CD27. Plays a role in T-cell activation. Induces the proliferation of costimulated T-cells and enhances the generation of cytolytic T-cells.

产品编号	Z04180
规格	100 μg 500 μg 大单询价
数量
价格	￥3500


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产品简介

Species

Human

Protein Construction

His	CD27 Ligand/CD70 Trimer (Leu50-Pro193) Accession # P32970-1
N-term	C-term

Purity

> 95% as determined by BisTris PAGE
> 95% as determined by HPLC

Endotoxin Level

Less than 1EU per μg by the LAL method.

Biological Activity

Measured by its binding ability in a functional ELISA. Immobilized CD27 Ligand/CD70 Trimer, His, Human at 2μg/ml (100μl/Well) on the plate can bind Human CD27, hFc Tag. Test result was comparable to standard batch.

Expression System

HEK293

Theoretical Molecular Weight

51.8 kDa

Apparent Molecular Weight

Due to glycosylation, the protein migrates to 62-65 kDa based on Bis-Tris PAGE result.

Formulation

Lyophilized from 0.22μm filtered solution in PBS (pH 7.4).

Reconstitution

Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water.

Storage & Stability

Upon receiving, the product remains stable up to 6 months at -20 °C or below. Upon reconstitution, the product should be stable for 3 months at -80 °C. Avoid repeated freeze-thaw cycles.

图例

Immobilized CD27 Ligand/CD70 Trimer, His, Human, His Tag at 2 μg/ml (100 μL/well) on the plate. Dose response curve for Human CD27, hFc Tag with the EC50 of 11.8 ng/ml determined by ELISA.

The purity of CD27 Ligand/CD70 Trimer, His, Human is greater than 95% as determined by SEC-HPLC.

CD27 Ligand/CD70 Trimer, His, Human on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.

技术背景

Target Background	CD70, also named CD27 ligand (CD27L), is a type II transmembrane glycoprotein belonging to the TNF superfamily (TNFSF) and has been designated TNFSF7. CD70 is a cytokine that binds to CD27. Plays a role in T-cell activation. Induces the proliferation of costimulated T-cells and enhances the generation of cytolytic T-cells.
Synonyms	CD70 molecule; CD70; TNFSF7; CD27 Ligand; CD27-L; CD27LG; Ki-24 antigen; TNFSF7G

For research use only. Not intended for human or animal clinical trials, therapeutic or diagnostic use.