Immobilized Human B7-H7, His Tag at 5 μg/ml (100 μL/well) on the plate. Dose response curve for CD28H/IGPR-1 hFc Chimera, Human, hFc Tag with the EC50 of 63.4 ng/ml determined by ELISA.

The purity of CD28H/IGPR-1 hFc Chimera, Human is greater than 95% as determined by SEC-HPLC.

CD28H/IGPR-1 hFc Chimera, Human on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.

CD28H/IGPR-1 hFc Chimera, Human

CD28H is constitutively expressed on all naive T cells. Repetitive antigenic exposure, however, induces a complete loss of CD28H on many T cells, and CD28H negative T cells have a phenotype of terminal differentiation and senescence. After extensive screening in a receptor array, a B7-like molecule, B7 homologue 5 (B7-H5), was identified as a specific ligand for CD28H.

产品编号	Z04184
规格	100 μg 500 μg 大单询价
数量
价格	￥3500


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产品简介

Species

Human

Protein Construction

CD28H/IGPR-1 (Leu23-Gly150) Accession # Q96BF3-1	hFc
N-term	C-term

Purity

> 95% as determined by BisTris PAGE
> 95% as determined by HPLC

Endotoxin Level

Less than 1EU per μg by the LAL method.

Biological Activity

Measured by its binding ability in a functional ELISA. Immobilized Human B7H7, His Tag at 5μg/ml (100μl/well) on the plate can bind CD28H/IGPR-1 hFc Chimera, Human. Test result was comparable to standard batch.

Expression System

HEK293

Theoretical Molecular Weight

40.7 kDa

Apparent Molecular Weight

Due to glycosylation, the protein migrates to 50-60 kDa based on Bis-Tris PAGE result.

Formulation

Lyophilized from 0.22μm filtered solution in PBS (pH 7.4).

Reconstitution

Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water.

Storage & Stability

Upon receiving, the product remains stable up to 6 months at -20 °C or below. Upon reconstitution, the product should be stable for 3 months at -80 °C. Avoid repeated freeze-thaw cycles.

图例

Immobilized Human B7-H7, His Tag at 5 μg/ml (100 μL/well) on the plate. Dose response curve for CD28H/IGPR-1 hFc Chimera, Human, hFc Tag with the EC50 of 63.4 ng/ml determined by ELISA.

The purity of CD28H/IGPR-1 hFc Chimera, Human is greater than 95% as determined by SEC-HPLC.

CD28H/IGPR-1 hFc Chimera, Human on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.

技术背景

Target Background	CD28H is constitutively expressed on all naive T cells. Repetitive antigenic exposure, however, induces a complete loss of CD28H on many T cells, and CD28H negative T cells have a phenotype of terminal differentiation and senescence. After extensive screening in a receptor array, a B7-like molecule, B7 homologue 5 (B7-H5), was identified as a specific ligand for CD28H.
Synonyms	TMIGD2; IGPR1; IGPR-1; CD28H; TPC1

For research use only. Not intended for human or animal clinical trials, therapeutic or diagnostic use.