| Species |
Mouse |
| Protein Construction |
CD8 alpha & beta Heterodimer [Lys28-Tyr196 (CD8 alpha) acidic tail and Leu22-Thr175 (CD8 beta) basic tail]
Accession # P01731 (CD8 alpha) & P10300 (CD8 beta) |
His |
Flag |
| N-term |
|
C-term |
|
| Purity |
> 95% as determined by Bis-Tris PAGE
> 95% as determined by HPLC |
| Endotoxin Level |
Less than 1EU per μg by the LAL method. |
| Expression System |
HEK293 |
| Theoretical Molecular Weight |
24.80 kDa (CD8 alpha) and 23.17 kDa (CD8 beta) |
| Apparent Molecular Weight |
Due to glycosylation, the protein migrates to 32-38 kDa (CD8 alpha) and 42-50 kDa (CD8 beta) based on Bis-Tris PAGE result. |
| Formulation |
Lyophilized from a 0.22 μm filtered solution in PBS, (pH 7.4). |
| Reconstitution |
Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water. |
| Storage & Stability |
Upon receiving, the product remains stable up to 6 months at -20 °C or below. Upon reconstitution, the product should be stable for 3 months at -80 °C. Avoid repeated freeze-thaw cycles. |
The purity of CD8 alpha&beta Heterodimer, His & Flag, Mouse is greater than 95% as determined by SEC-HPLC.
CD8 alpha&beta Heterodimer, His & Flag, Mouse on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
| Target Background |
CD8 alpha&beta (CD8αβ) is a heterodimeric form of CD8. CD8α is required for surface expression of CD8β. The extracellular IgV-like domain of CD8α interacts with the α3 portion of the class I MHC molecule. CD8αβ is expressed on human peripheral T cells and functions as a coreceptor and can greatly increase the sensitivity and breadth of antigen recognition by CD8+ peripheral T cells bearing TCR. |
| Synonyms |
CD8 alphaβ CD8A&CD8B; |
For research use only. Not intended for human or animal clinical trials, therapeutic or diagnostic use.
