Immobilized IL-2 R alpha/CD25, His, Mouse, His Tag at 5 μg/ml (100 μL/well) on the plate. Dose response curve for Biotinylated Mouse IL-2, His Tag with the EC50 of 0.17 μg/ml determined by ELISA.

The purity of IL-2 R alpha/CD25, His, Mouse is greater than 95% as determined by SEC-HPLC.

IL-2 R alpha/CD25, His, Mouse on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.

IL-2 R alpha/CD25, His, Mouse

Human IL-2 receptor alpha (IL-2 R alpha), also known as Tac antigen and as CD25, was initially identified as a 55 kDa membrane glycoprotein that is capable of binding IL‑2.eceptor for interleukin-2. The receptor is involved in the regulation of immune tolerance by controlling regulatory T cells (TREGs) activity. TREGs suppress the activation and expansion of autoreactive T-cells.

产品编号	Z04342
规格	100 μg 500 μg 大单询价
数量
价格	￥3300


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产品简介

Species

Mouse

Protein Construction

IL-2 R alpha/CD25 (Glu22-Lys236) Accession # P01590	His
N-term	C-term

Purity

> 95% as determined by BisTris PAGE
> 95% as determined by HPLC

Endotoxin Level

Less than 1EU per μg by the LAL method.

Biological Activity

Measured by its binding ability in a functional ELISA. Immobilized IL-2 R alpha/CD25, His, Mouse at 5μg/ml (100μl/well) on the plate can bind Biotinylated Mouse IL2, His Tag. Test result was comparable to standard batch.

Expression System

HEK293

Theoretical Molecular Weight

25.76 kDa

Apparent Molecular Weight

Due to glycosylation, the protein migrates to 50-70 kDa based on Bis-Tris PAGE result.

Formulation

Lyophilized from 0.22μm filtered solution in PBS (pH 7.4).

Reconstitution

Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water.

Storage & Stability

Upon receiving, the product remains stable up to 6 months at -20 °C or below. Upon reconstitution, the product should be stable for 3 months at -80 °C. Avoid repeated freeze-thaw cycles.

图例

Immobilized IL-2 R alpha/CD25, His, Mouse, His Tag at 5 μg/ml (100 μL/well) on the plate. Dose response curve for Biotinylated Mouse IL-2, His Tag with the EC50 of 0.17 μg/ml determined by ELISA.

The purity of IL-2 R alpha/CD25, His, Mouse is greater than 95% as determined by SEC-HPLC.

IL-2 R alpha/CD25, His, Mouse on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.

技术背景

Target Background	Human IL-2 receptor alpha (IL-2 R alpha), also known as Tac antigen and as CD25, was initially identified as a 55 kDa membrane glycoprotein that is capable of binding IL‑2.eceptor for interleukin-2. The receptor is involved in the regulation of immune tolerance by controlling regulatory T cells (TREGs) activity. TREGs suppress the activation and expansion of autoreactive T-cells.
Synonyms	CD25; IDDM10; IL-2 R alpha; IL2R; IL2RA; p55; TCGFR

For research use only. Not intended for human or animal clinical trials, therapeutic or diagnostic use.