| Species |
Human |
| Protein Construction |
HMGB1 (Met1-Glu215)
Accession # P09429-1 |
His |
| N-term |
C-term |
|
| Conjugate |
Biotin |
| Purity |
> 95% as determined by BisTris PAGE
> 95% as determined by HPLC |
| Endotoxin Level |
Less than 1EU per ug by the LAL method. |
| Expression System |
HEK293 |
| Theoretical Molecular Weight |
26 kDa |
| Apparent Molecular Weight |
Due to glycosylation, the protein migrates to 32-36 kDa based on Bis-Tris PAGE result. |
| Formulation |
Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). |
| Reconstitution |
Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water. |
| Storage & Stability |
Upon receiving, the product remains stable up to 6 months at -20 °C or below. Upon reconstitution, the product should be stable for 3 months at -80 °C. Avoid repeated freeze-thaw cycles. |
| Target Background |
High-mobility group box 1 (HMGB1) is a ubiquitous nuclear protein that promotes inflammation when released extracellularly after cellular activation, stress, damage or death. HMGB1 operates as one of the most intriguing molecules in inflammatory disorders via recently elucidated signal and molecular transport mechanisms. Treatments based on antagonists specifically targeting extracellular HMGB1 have generated encouraging results in a wide number of experimental models of infectious and sterile inflammation. |
| Synonyms |
HMGB1; HMG1; HMG3; SBP-1; HMG-1 |
For research use only. Not intended for human or animal clinical trials, therapeutic or diagnostic use.
