| Species |
Human |
| Protein Construction |
IL-15RA & IL-15 [Ile31-Ser108 (IL-15RA) & Asn49-Ser162 (IL-15)]
Accession # Q13261-1 (IL-15RA) & P40933-1 (IL-15) |
His |
| N-term |
C-term |
|
| Conjugate |
Biotin |
| Purity |
> 95% as determined by Bis-Tris PAGE |
| Endotoxin Level |
Less than 1 EU per ug by the LAL method. |
| Biological Activity |
Measured by its binding ability in a functional ELISA. Immobilized IL-15RA&IL-15 [Biotin], His, Human at 1μg/ml (100μl/well) on the streptavidin precoated plate (5 μg/ml) can bind Human IL2 R beta, hFc Tag. Test result was comparable to standard batch. |
| Expression System |
HEK293 |
| Theoretical Molecular Weight |
23.8 kDa |
| Apparent Molecular Weight |
Due to glycosylation, the protein migrates to 25-28 kDa&30-35 kDa based on Bis-Tris PAGE result. |
| Formulation |
Lyophilized from 0.22 μm filtered solution in PBS (pH 7.4). |
| Reconstitution |
Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water. |
| Storage & Stability |
Upon receiving, the product remains stable up to 6 months at -20 °C or below. Upon reconstitution, the product should be stable for 3 months at -80 °C. Avoid repeated freeze-thaw cycles. |
| Target Background |
Interleukin-15 receptor alpha (IL-15R alpha) is a high affinity IL-15 binding protein that is crucial for mediating IL-15 functions such as memory CD8 T cell proliferation and NK, NK/T cell, and intestinal intraepithelial lymphocyte development. |
| Synonyms |
IL-15 R alpha;CD215;IL15RA;IL-15RA;IL-15R-alpha1;Interleukin-15;IL-15;IL15; Nanrilkefusp alfa |
For research use only. Not intended for human or animal clinical trials, therapeutic or diagnostic use.
