| Species |
Human |
| Protein Construction |
Mature TGF beta 1 (Ala279-Ser390)
Accession # P01137 |
Avi |
| N-term |
C-term |
|
| Conjugate |
Biotin |
| Purity |
> 95% as determined by Bis-Tris PAGE |
| Endotoxin Level |
Less than 1EU per μg by the LAL method. |
| Biological Activity |
Measured by its binding ability in a functional ELISA. Immobilized Human TGFbeta RII, hFc Tag at 1μg/ml (100μl/well) on the plate can bind Mature TGF beta 1[Biotin], Avi, Human. Test result was comparable to standard batch. |
| Expression System |
HEK293 |
| Theoretical Molecular Weight |
13.2 kDa |
| Apparent Molecular Weight |
Due to glycosylation, the protein migrates to 15-18 kDa under reduced (R) condition and 26-30 kDa under Non reducing (N) condition based on Bis-Tris PAGE result. |
| Formulation |
Lyophilized from 0.22μm filtered solution in 50mM Glycine, 150mM NaCl (pH 2.5). |
| Reconstitution |
Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in 4mM HCl. |
| Storage & Stability |
Upon receiving, the product remains stable up to 6 months at -20 °C or below. Upon reconstitution, the product should be stable for 3 months at -80 °C. Avoid repeated freeze-thaw cycles. |
| Target Background |
TGF-beta 1 (transforming growth factor beta 1) is one of three closely related mammalian members of the large TGF-beta superfamily that share a characteristic cystine knot structure. TGF-beta 1, -2 and -3 are highly pleiotropic cytokines that are proposed to act as cellular switches that regulate processes such as immune function, proliferation and epithelial-mesenchymal transition.Transforming growth factor beta-1 is multifunctional protein that regulates the growth and differentiation of various cell types and is involved in various processes, such as normal development, immune function, microglia function and responses to neurodegeneration. |
| Synonyms |
CEDLAP; DPD1; TGF beta1; TGFB; TGFB1; TGFbeta; TGF-beta-1; TGF β1; TGFβ; TGF-β-1 |
For research use only. Not intended for human or animal clinical trials, therapeutic or diagnostic use.
