| Species |
Human |
| Protein Construction |
Siglec-4a/MAG (Gly20-Pro516)
Accession # P20916 |
His |
Avi |
| N-term |
|
C-term |
|
| Conjugate |
Biotin |
| Purity |
> 95% as determined by BisTris PAGE
> 95% as determined by HPLC |
| Endotoxin Level |
Less than 1EU per μg by the LAL method. |
| Biological Activity |
Measured by its binding ability in a functional ELISA. Immobilized AntiSiglec4a Antibody, hFc Tag at 2μg/ml (100μl/well) on the plate can bind Siglec-4a/MAG[Biotin], His & Avi, Human. Test result was comparable to standard batch. |
| Expression System |
HEK293 |
| Theoretical Molecular Weight |
57.6 kDa |
| Apparent Molecular Weight |
Due to glycosylation, the protein migrates to 78-85 kDa based on Bis-Tris PAGE result. |
| Formulation |
Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). |
| Reconstitution |
Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water. |
| Storage & Stability |
Upon receiving, the product remains stable up to 6 months at -20 °C or below. Upon reconstitution, the product should be stable for 3 months at -80 °C. Avoid repeated freeze-thaw cycles. |
| Target Background |
Siglec-4a, also known as Myelin-Associated Glycoprotein (MAG), is a type I transmembrane glycoprotein belonging to the Siglec family, a subgroup of the Ig superfamily. Adhesion molecule that mediates interactions between myelinating cells and neurons by binding to neuronal sialic acid-containing gangliosides and to the glycoproteins RTN4R and RTN4RL2 (By similarity). |
| Synonyms |
MAG; Siglec4a; Siglec-4a; S-MAG; GMA |
For research use only. Not intended for human or animal clinical trials, therapeutic or diagnostic use.
