| Species |
Human |
| Protein Construction |
| Avi |
His |
Transferrin R/CD71 (Cys89-Phe760)
Accession # P02786 |
| N-term |
|
C-term |
|
| Conjugate |
Biotin |
| Purity |
> 95% as determined by Bis-Tris PAGE |
| Endotoxin Level |
Less than 1EU per μg by the LAL method. |
| Biological Activity |
Measured by its binding ability in a functional ELISA. Immobilized Transferrin R/CD71[Biotin], His & Avi, Human at 0.5μg/ml (100μl/well) on the streptavidin precoated plate (5μg/ml) can bind AntiTransferrin R Antibody, hFc Tag. Test result was comparable to standard batch. |
| Expression System |
HEK293 |
| Theoretical Molecular Weight |
77.9 kDa |
| Apparent Molecular Weight |
Due to glycosylation, the protein migrates to 80-85 kDa based on Bis-Tris PAGE result. |
| Formulation |
Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). |
| Reconstitution |
Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water. |
| Storage & Stability |
Upon receiving, the product remains stable up to 6 months at -20 °C or below. Upon reconstitution, the product should be stable for 3 months at -80 °C. Avoid repeated freeze-thaw cycles. |
| Target Background |
The transferrin receptor (CD71) is known as a receptor for IgA1 on mesangial cells. The transcript and protein expression of glomerular CD71 was determined by real-time polymerase chain reaction and immunohistochemistry. Human mesangial cells (HMCs) were treated with sera from IgAN patients and expression levels of CD71 and inflammatory cytokine markers were compared according to disease status. |
| Synonyms |
TR; TfR1; CD71; sTfR; T9; p90; TFRC; TFR |
For research use only. Not intended for human or animal clinical trials, therapeutic or diagnostic use.
