| Species |
Human |
| Protein Construction |
ULBP-1 (Gly26-Pro215)
Accession # Q9BZM6 |
His |
Avi |
| N-term |
|
C-term |
|
| Conjugate |
Biotin |
| Purity |
> 95% as determined by BisTris PAGE
> 95% as determined by HPLC |
| Endotoxin Level |
Less than 1EU per μg by the LAL method. |
| Biological Activity |
Measured by its binding ability in a functional ELISA. Immobilized Human NKG2D, hFc Tag at 2μg/ml (100μl/well) on the plate can bind ULBP-1[Biotin], His & Avi, Human. Test result was comparable to standard batch. |
| Expression System |
HEK293 |
| Theoretical Molecular Weight |
25.2 kDa |
| Apparent Molecular Weight |
Due to glycosylation, the protein migrates to 28-35 kDa based on Bis-Tris PAGE result. |
| Formulation |
Lyophilized from 0.22 μm filtered solution in 20mM PB, 0.5M NaCl, 0.1M L-arginine (pH 8.0). |
| Reconstitution |
Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water. |
| Storage & Stability |
Upon receiving, the product remains stable up to 6 months at -20 °C or below. Upon reconstitution, the product should be stable for 3 months at -80 °C. Avoid repeated freeze-thaw cycles. |
| Target Background |
Cell surface proteins major histocompatibility complex (MHC) class I-related chain A (MICA) and UL16-binding proteins (ULBP) 1, 2, and 3 are up-regulated upon infection or tumor transformation and can activate human natural killer (NK) cells. |
| Synonyms |
ALCAN-beta; NKG2D ligand 1; N2DL-1; NKG2DL1; MULT-1; NKG2DL; AET1I; ULBP1; ULBP-1;ALCAN-β |
For research use only. Not intended for human or animal clinical trials, therapeutic or diagnostic use.
