| Species |
Canine |
| Protein Construction |
| His |
PDGFA (Ser87-Arg196)
Accession # A0A8C0M6T8 |
| N-term |
C-term |
|
| Purity |
> 90% as determined by Bis-Tris PAGE |
| Endotoxin Level |
Less than 1EU per μg by the LAL method. |
| Biological Activity |
Measured in a cell proliferation assay using Balb/C 3T3 mouse embryonic fibroblasts. The ED50 for this effect is 5 15 ng/mL. Test result was comparable to standard batch. |
| Expression System |
Yeast |
| Theoretical Molecular Weight |
13.62 kDa |
| Apparent Molecular Weight |
Due to glycosylation, the protein migrates to 15-20 kDa and 35-45 kDa based on Bis-Tris PAGE result. |
| Formulation |
Lyophilized from 0.22μm filtered solution in 4mM HCL. |
| Reconstitution |
Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in 4mM HCL. |
| Storage & Stability |
Upon receiving, the product remains stable up to 6 months at -20 °C or below. Upon reconstitution, the product should be stable for 3 months at -80 °C. Avoid repeated freeze-thaw cycles. |
| Target Background |
Platelet-derived growth factors (PDGFs) may play an important role in the development of atherosclerosis acting as chemoattractants and mitogens for vascular smooth muscle cells and macrophages. PDGFA, the most known member of PDGF family, plays a crucial role in occurrence and progression of different tumors. |
| Synonyms |
PDGF-AA; PDGF-A; PDGF1; PDGF A; PDGFAA; PDGF AA |
For research use only. Not intended for human or animal clinical trials, therapeutic or diagnostic use.
