| Species |
Cynomolgus |
| Protein Construction |
B7-H6/NCR3LG1 (Asp25-Asp259)
Accession # XP_005578557.1 |
His |
| N-term |
C-term |
|
| Purity |
> 95% as determined by BisTris PAGE
> 95% as determined by HPLC |
| Endotoxin Level |
Less than 1 EU per μg by the LAL method. |
| Biological Activity |
Human NKp30, hFc Tag captured on CM5 Chip via Protein A can bind B7-H6/NCR3LG1, His, Cynomolgus in SPR assay (Biacore T200). Test result was comparable to standard batch. |
| Expression System |
HEK293 |
| Theoretical Molecular Weight |
27.6 kDa |
| Apparent Molecular Weight |
Due to glycosylation, the protein migrates to 40-52 kDa based on Bis-Tris PAGE result. |
| Formulation |
Lyophilized from 0.22 μm filtered solution in PBS (pH 7.4). |
| Reconstitution |
Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water. |
| Storage & Stability |
Upon receiving, the product remains stable up to 6 months at -20 °C or below. Upon reconstitution, the product should be stable for 3 months at -80 °C. Avoid repeated freeze-thaw cycles. |
| Target Background |
B7-H6 is a glycosylated member of the B7 family of immune co‑stimulatory proteins, which is a ligand for the NK cell activating receptor NKp30, was targeted to create a CAR that targets multiple tumor types. B7-H6 is expressed on various primary human tumors, including leukemia, lymphoma and gastrointestinal stromal tumors, but it is not constitutively expressed on normal tissues. |
| Synonyms |
B7H6; B7-H6; DKFZp686I21167; DKFZp686O24166; NCR3LG1; B7 Homolog 6 |
For research use only. Not intended for human or animal clinical trials, therapeutic or diagnostic use.
