| Species |
Cynomolgus |
| Protein Construction |
IFN alpha/beta R2 (Ile27-Lys243)
Accession # EHH53981.1 |
His |
| N-term |
C-term |
|
| Purity |
> 95% as determined by BisTris PAGE
> 95% as determined by HPLC |
| Endotoxin Level |
Less than 1EU per μg by the LAL method. |
| Biological Activity |
IFN alpha/beta R2, His, Cynomolgus captured on CM5 Chip via antihis antibody can bind Human IFN alpha 1, hFc Tag in SPR assay (Biacore T200). Test result was comparable to standard batch. |
| Expression System |
HEK293 |
| Theoretical Molecular Weight |
25.93 kDa |
| Apparent Molecular Weight |
Due to glycosylation, the protein migrates to 43-60 kDa based on Bis-Tris PAGE result. |
| Formulation |
Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). |
| Reconstitution |
Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water. |
| Storage & Stability |
Upon receiving, the product remains stable up to 6 months at -20 °C or below. Upon reconstitution, the product should be stable for 3 months at -80 °C. Avoid repeated freeze-thaw cycles. |
| Target Background |
Although interferon (IFN)-α is known to exert immunomodulatory and antiproliferative effects on dendritic cells (DCs) through induction of protein-coding IFN-stimulated genes (ISGs), little is known about IFN-α-regulated miRNAs in DCs. Since several miRNAs are involved in regulating DC functions, it is important to investigate whether IFN-α's effects on DCs are mediated through miRNAs as well. |
| Synonyms |
IFN-R-2; IFNAR2; IFNARB; IFNABR; IFN-alpha; IFN-alpha/beta R2; IFNbR2; IFN-R; β receptor 2; β receptor10; |
For research use only. Not intended for human or animal clinical trials, therapeutic or diagnostic use.
