| Species |
Cynomolgus |
| Protein Construction |
IL-1R3/IL-1 RAcP (Ser21-Cys362)
Accession # G7NYP7 |
His |
| N-term |
C-term |
|
| Purity |
> 95% as determined by BisTris PAGE
> 95% as determined by HPLC |
| Endotoxin Level |
Less than 1 EU per μg by the LAL method. |
| Biological Activity |
Measured by its binding ability in a functional ELISA. Immobilized IL-1R3/IL-1 RAcP, His, Cynomolgus at 0.5 μg/ml (100μl/well) on the plate can bind AntiIL1R3 Antibody, hFc Tag. Test result was comparable to standard batch. |
| Expression System |
HEK293 |
| Theoretical Molecular Weight |
40.49 kDa |
| Apparent Molecular Weight |
Due to glycosylation, the protein migrates to 55-70 kDa based on Bis-Tris PAGE result. |
| Formulation |
Lyophilized from 0.22 μm filtered solution in PBS (pH 7.4). |
| Reconstitution |
Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water. |
| Storage & Stability |
Upon receiving, the product remains stable up to 6 months at -20 °C or below. Upon reconstitution, the product should be stable for 3 months at -80 °C. Avoid repeated freeze-thaw cycles. |
| Target Background |
IL-1 Receptor Accessory Protein (IL-1 RAcP), also known as IL-1 R3, is a ubiquitously expressed 70-90 kDa member of the Interleukin-1 receptor family of proteins. It serves as a non-ligand-binding component of the receptors for IL-1 alpha, IL-1 beta, IL-33, and IL-36. IL-1 R3 is a coreceptor for IL1RL2 in the IL-36 signaling system. IL-1 R3 is a coreceptor with IL1R1 in the IL-1 signaling system. Associates with IL1R1 bound to IL1B to form the high affinity interleukin-1 receptor complex which mediates interleukin-1-dependent activation of NF-kappa-B and other pathways. |
| Synonyms |
IL1RAP; IL-1RAP; C3orf13; IL-1 R3; IL-1 RAcP |
For research use only. Not intended for human or animal clinical trials, therapeutic or diagnostic use.
