| Species |
Human |
| Protein Construction |
Alkaline Phosphatase (Germ type)/ALPG (Ile20-Asp503)
Accession # P10696 |
His |
| N-term |
C-term |
|
| Purity |
> 95% as determined by BisTris PAGE
> 95% as determined by HPLC |
| Endotoxin Level |
Less than 1EU per μg by the LAL method. |
| Biological Activity |
Measured by its binding ability in a functional ELISA. Immobilized Alkaline Phosphatase (Germ type)/ALPG, His, Human at 1 μg/ml (100 μl/well) on the plate can bind AntiAlkaline Phosphatase Antibody, hFc Tag. Test result was comparable to standard batch. |
| Expression System |
HEK293 |
| Theoretical Molecular Weight |
53.67 kDa |
| Apparent Molecular Weight |
Due to glycosylation, the protein migrates to 58-68 kDa based on Bis-Tris PAGE result. |
| Formulation |
Lyophilized from 0.22 μm filtered solution in 25 mM Tris, 100 mM NaCl, 1 mM MgCl2 (pH 7.5). |
| Reconstitution |
Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water. |
| Storage & Stability |
Upon receiving, the product remains stable up to 6 months at -20 °C or below. Upon reconstitution, the product should be stable for 3 months at -80 °C. Avoid repeated freeze-thaw cycles. |
| Target Background |
Alkaline phosphatase can be considered 'our favorite enzyme' for reasons apparent to those who diagnose and treat metabolic bone diseases or who study skeletal biology. Few might know, however, that alkaline phosphatase likely represents the most frequently assayed enzyme in all of medicine. Elevated activity in the circulation is universally recognized as a marker for skeletal or hepatobiliary disease. |
| Synonyms |
Alkaline Phosphatase; ALP-1; Alkaline phosphatase, placental-like; GCAP; PLAP-like; ALPG; ALPPL; ALPPL2 |
For research use only. Not intended for human or animal clinical trials, therapeutic or diagnostic use.
