| Species |
Human |
| Protein Construction |
BCHE/Butyrylcholinesterase (Glu29-Leu602)
Accession # P06276 |
His |
| N-term |
C-term |
|
| Purity |
> 95% as determined by Bis-Tris PAGE |
| Endotoxin Level |
Less than 1EU per μg by the LAL method. |
| Biological Activity |
Measured by its ability to cleave Butyrylthiocholine. The specific activity is > 70000 pmoles/min/μg. Test result meets the standard. |
| Expression System |
HEK293 |
| Theoretical Molecular Weight |
66.18 kDa |
| Apparent Molecular Weight |
Due to glycosylation, the protein migrates to 70-110 kDa based on Bis-Tris PAGE result. |
| Formulation |
Lyophilized from 0.22 μm filtered solution in 50mM Tris, 150mM Nacl (pH 8.0). |
| Reconstitution |
Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water. |
| Storage & Stability |
Upon receiving, the product remains stable up to 6 months at -20 °C or below. Upon reconstitution, the product should be stable for 3 months at -80 °C. Avoid repeated freeze-thaw cycles. |
| Target Background |
Butyrylcholinesterase is a serine hydrolase biochemically related to the cholinergic enzyme acetylcholinesterase. It is capable of hydrolyzing esters of choline. Butyrylcholinesterase has unique enzymatic properties and is widely distributed in the nervous system, raising the possibility of its involvement in neural function. |
| Synonyms |
Cholinesterase; BCHE; CHE1 |
For research use only. Not intended for human or animal clinical trials, therapeutic or diagnostic use.
