| Species |
Human |
| Protein Construction |
CA12/Carbonic anhydrase XII (Ala25-Ser301)
Accession # O43570-1 |
His |
| N-term |
C-term |
|
| Purity |
> 95% as determined by BisTris PAGE
> 95% as determined by HPLC |
| Endotoxin Level |
Less than 1EU per μg by the LAL method. |
| Biological Activity |
Measured by its esterase activity. The specific activity is > 40 pmol/min/µg. Test result meets the standard. |
| Expression System |
HEK293 |
| Theoretical Molecular Weight |
32.22 kDa |
| Apparent Molecular Weight |
Due to glycosylation, the protein migrates to 40-50 kDa based on Bis-Tris PAGE result. |
| Formulation |
Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). |
| Reconstitution |
Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water. |
| Storage & Stability |
Upon receiving, the product remains stable up to 6 months at -20 °C or below. Upon reconstitution, the product should be stable for 3 months at -80 °C. Avoid repeated freeze-thaw cycles. |
| Target Background |
Carbonic anhydrases (CAs) are a family of enzymes involved in the pH regulation of metabolically active cells/tissues. Carbonic anhydrase XII (CA XII) is a key mediator of several signaling pathways that are involved in cancer development. |
| Synonyms |
Carbonic anhydrase; CA12; CAXII; Carbonic anhydrase 12; CA-XII; Tumor antigen HOM-RCC-3.1.3 |
For research use only. Not intended for human or animal clinical trials, therapeutic or diagnostic use.
