| Species |
Human |
| Protein Construction |
DSG-2/Desmoglein-2 (Ala50-Gly609)
Accession # Q14126 |
His |
| N-term |
C-term |
|
| Purity |
> 95% as determined by Bis-Tris PAGE |
| Endotoxin Level |
Less than 1EU per μg by the LAL method. |
| Biological Activity |
Measured by its binding ability in a functional ELISA. Immobilized DSG-2/Desmoglein-2, His, Human at 1μg/ml (100μl/well) on the plate can bind AntiDSG2 Antibody, hFc Tag. Test result was comparable to standard batch. |
| Expression System |
HEK293 |
| Theoretical Molecular Weight |
63.6 kDa |
| Apparent Molecular Weight |
Due to glycosylation, the protein migrates to 75-80 kDa based on Bis-Tris PAGE result. |
| Formulation |
Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). |
| Reconstitution |
Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water. |
| Storage & Stability |
Upon receiving, the product remains stable up to 6 months at -20 °C or below. Upon reconstitution, the product should be stable for 3 months at -80 °C. Avoid repeated freeze-thaw cycles. |
| Target Background |
Desmoglein-2 (DSG-2) as the primary high-affinity receptor used by adenoviruses Ad3, Ad7, Ad11 and Ad14. These serotypes represent key human pathogens causing respiratory and urinary tract infections. In epithelial cells, adenovirus binding of DSG-2 triggers events reminiscent of epithelial-to-mesenchymal transition, leading to transient opening of intercellular junctions. |
| Synonyms |
Desmoglein-2; HDGC; DSG2; CDHF5; ARVC10; CMD1BB; MGC117036; MGC117037 |
For research use only. Not intended for human or animal clinical trials, therapeutic or diagnostic use.
