| Species |
Human |
| Protein Construction |
| His |
ENPP-2/Autotaxin (Asp49-Ile863)
Accession # NP_001035181 |
| N-term |
C-term |
|
| Purity |
> 95% as determined by BisTris PAGE
> 95% as determined by HPLC |
| Endotoxin Level |
Less than 1EU per μg by the LAL method. |
| Biological Activity |
Measured by its ability to cleave Bis (pNitrophenyl) Phosphate (BPNPP). The specific activity is > 10000 pmol/min/μg. Test result meets the standard. |
| Expression System |
HEK293 |
| Theoretical Molecular Weight |
94.8 kDa |
| Apparent Molecular Weight |
Due to glycosylation, the protein migrates to 95-115 kDa based on Bis-Tris PAGE result. |
| Formulation |
Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). |
| Reconstitution |
Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water. |
| Storage & Stability |
Upon receiving, the product remains stable up to 6 months at -20 °C or below. Upon reconstitution, the product should be stable for 3 months at -80 °C. Avoid repeated freeze-thaw cycles. |
| Target Background |
Ectonucleotide pyrophosphatase/phosphodiesterase 2 (ENPP2) also known as Autotaxin, is a secreted lysophospholipase D, which hydrolyzes lysophosphatidylcholine (LPC) into Lysophosphatidic acid (LPA). ENPP2 is an essential protein for normal development and its altered expression is associated with various human diseases. |
| Synonyms |
PDNP2; ATX-X; NPP2; PD-IALPHA; ENPP-2; E-NPP 2; Autotaxin; LysoPLD; ATX |
For research use only. Not intended for human or animal clinical trials, therapeutic or diagnostic use.
