The purity of Fc gamma RIIIB/CD16b (NA1), His, Human is greater than 95% as determined by SEC-HPLC.

Fc gamma RIIIB/CD16b (NA1), His, Human on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.

Fc gamma RIIIB/CD16b (NA1), His, Human, His Tag captured on CM5 Chip via anti-his antibody can bind Rituximab with an affinity constant of 8.55 μM as determined in SPR assay (Biacore T200).

Fc gamma RIIIB/CD16b (NA1), His, Human

Human Fc gamma RIIIB/CD16b Protein is a receptor for the Fc region of immunoglobulins gamma. Low affinity receptor. Binds complexed or aggregated IgG and also monomeric IgG. Contrary to III-A, is not capable to mediate antibody-dependent cytotoxicity and phagocytosis. May serve as a trap for immune complexes in the peripheral circulation which does not activate neutrophils.

产品编号	Z05279
规格	100 μg 500 μg 大单询价
数量
价格	￥2600


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产品简介

Species

Human

Protein Construction

Fc gamma RIIIB/CD16b (NA1) [Gly17-Ser200 (NA1)] Accession # AAA35881.1	His
N-term	C-term

Purity

> 95% as determined by Bis-Tris PAGE
> 95% as determined by HPLC

Endotoxin Level

Less than 1EU per μg by the LAL method.

Biological Activity

Fc gamma RIIIB/CD16b (NA1), His, Human captured on CM5 Chip via antihis antibody can bind Rituximab in SPR assay (Biacore T200). Test result was comparable to standard batch.

Expression System

HEK293

Theoretical Molecular Weight

22.52 kDa

Apparent Molecular Weight

Due to glycosylation, the protein migrates to 40-50 kDa based on Bis-Tris PAGE result.

Formulation

Lyophilized from a 0.22 μm filtered solution in PBS, (pH 7.4).

Reconstitution

Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water.

Storage & Stability

Upon receiving, the product remains stable up to 6 months at -20 °C or below. Upon reconstitution, the product should be stable for 3 months at -80 °C. Avoid repeated freeze-thaw cycles.

图例

The purity of Fc gamma RIIIB/CD16b (NA1), His, Human is greater than 95% as determined by SEC-HPLC.

Fc gamma RIIIB/CD16b (NA1), His, Human on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.

Fc gamma RIIIB/CD16b (NA1), His, Human, His Tag captured on CM5 Chip via anti-his antibody can bind Rituximab with an affinity constant of 8.55 μM as determined in SPR assay (Biacore T200).

技术背景

Target Background	Human Fc gamma RIIIB/CD16b Protein is a receptor for the Fc region of immunoglobulins gamma. Low affinity receptor. Binds complexed or aggregated IgG and also monomeric IgG. Contrary to III-A, is not capable to mediate antibody-dependent cytotoxicity and phagocytosis. May serve as a trap for immune complexes in the peripheral circulation which does not activate neutrophils.
Synonyms	CD16; Fc gamma RIIIB; FCG3; FCGR3B; FcgRIIIB; FCRIIIB; IGFR3; CD16b (NA2); CD16B; FCG3B; FCG3; FCGR3; FCR-10

For research use only. Not intended for human or animal clinical trials, therapeutic or diagnostic use.