| Species |
Human |
| Protein Construction |
GM-CSF (Ala18-Glu144)
Accession # P04141 |
|
| Purity |
> 95% as determined by BisTris PAGE
> 95% as determined by HPLC |
| Endotoxin Level |
Less than 0.1EU per μg by the LAL method. |
| Biological Activity |
Measured by its binding ability in a functional ELISA. Immobilized GM-CSF, Human at 2μg/ml (100μl/Well) on the plate can bind Human GMCSF R alpha, hFc Tag. Test result was comparable to standard batch. The ED50 as determined by the dosedependent stimulation of the proliferation of human TF1 cells is 0.020.1ng/mL. Test result was comparable to standard batch. |
| Expression System |
E.coli |
| Theoretical Molecular Weight |
14.6 kDa |
| Apparent Molecular Weight |
The protein has a predicted MW of 14.6 kDa same as Bis-Tris PAGE result. |
| Formulation |
Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). |
| Reconstitution |
Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water. |
| Storage & Stability |
Upon receiving, the product remains stable up to 6 months at -20 °C or below. Upon reconstitution, the product should be stable for 3 months at -80 °C. Avoid repeated freeze-thaw cycles. |
| Target Background |
Granulocyte-macrophage colony-stimulating factor (GM-CSF), also known as colony-stimulating factor 2 (CSF2), is a monomeric glycoprotein secreted by macrophages, T cells, mast cells, natural killer cells, endothelial cells and fibroblasts that functions as a cytokine. The pharmaceutical analogs of naturally occurring GM-CSF are called sargramostim and molgramostim. |
| Synonyms |
CSF; CSF2; GMCSF; GM-CSF; MGC131935; MGC138897 |
For research use only. Not intended for human or animal clinical trials, therapeutic or diagnostic use.
