| Species |
Human |
| Protein Construction |
gp130/CD130/IL-6 R beta (Glu23-Glu619)_x000D_
Accession # P40189-1 |
His |
| N-term |
C-term |
|
| Purity |
> 95% as determined by BisTris PAGE
> 95% as determined by HPLC |
| Endotoxin Level |
Less than 1EU per μg by the LAL method. |
| Biological Activity |
Measured by its binding ability in a functional ELISA. Immobilized Human IL6 R alpha, hFc Tag at 2μg/ml (100μl/well) on the Human IL6, No Tag precoated plate (5μg/ml) can bind gp130/CD130/IL-6 R beta, His, Human. Test result was comparable to standard batch. |
| Expression System |
HEK293 |
| Theoretical Molecular Weight |
68.94 kDa |
| Apparent Molecular Weight |
Due to glycosylation, the protein migrates to 80-110 kDa based on Bis-Tris PAGE result. |
| Formulation |
Lyophilized from 0.22 μm filtered solution in PBS (pH 7.4). |
| Reconstitution |
Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water. |
| Storage & Stability |
Upon receiving, the product remains stable up to 6 months at -20 °C or below. Upon reconstitution, the product should be stable for 3 months at -80 °C. Avoid repeated freeze-thaw cycles. |
| Target Background |
gp130 is a common signal transducing component of the functional receptor complexes for the interleukin (IL)-6 family of cytokines, ie, IL-6, IL-11, leukemia inhibitory factor (LIF), oncostatin M, ciliary neurotrophic factor, and cardiotrophin-1. These cytokines exhibit pleiotropic biological activities in, for instance, immune, hematopoietic, and neural systems, and function in a redundant manner owing to the shared usage of gp130. |
| Synonyms |
gp130; CD130; IL-6 R beta; IL-6R-beta; IL-6RB; CDw130; IL6ST |
For research use only. Not intended for human or animal clinical trials, therapeutic or diagnostic use.
