| Species |
Human |
| Protein Construction |
IL-15 (Asn49-Ser162)
Accession # P40933 |
|
| Purity |
> 95% as determined by BisTris PAGE
> 95% as determined by HPLC |
| Endotoxin Level |
Less than 0.05EU per μg by the LAL method. |
| Biological Activity |
Measured in a cell proliferation assay using CTLL2 cells. The ED50 for this effect is 0.1 0.3 ng/mL. The specific activity of recombinant IL-15, Human is > 1 x 10^7 units/mg, which is calibrated against the IL-15, Human reference standard (NIBSC code: 95/554). Test result was comparable to standard batch. |
| Expression System |
E.coli |
| Theoretical Molecular Weight |
12.8 kDa |
| Apparent Molecular Weight |
The protein has a predicted MW of 12.8 kDa same as Bis-Tris PAGE result. |
| Formulation |
Lyophilized from 0.22 μm filtered solution in 20 mM Tris, 150 mM NACI (pH 7.5). |
| Reconstitution |
Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water. |
| Storage & Stability |
Upon receiving, the product remains stable up to 6 months at -20 °C or below. Upon reconstitution, the product should be stable for 3 months at -80 °C. Avoid repeated freeze-thaw cycles. |
| Target Background |
Interleukin 15 (IL-15) is a widely expressed 14 kDa cytokine that is structurally and functionally related to IL-2 and plays an important role in many immunological diseases. IL-15 is a cytokine that stimulates the proliferation of T-lymphocytes. Stimulation by IL15 requires interaction of IL15 with components of the IL2 receptor, including IL2RB and probably IL2RG but not IL2RA. |
| Synonyms |
L15; IL-15; IL-15MGC9721; Interleukin-15 |
For research use only. Not intended for human or animal clinical trials, therapeutic or diagnostic use.
