| Species |
Human |
| Protein Construction |
IL-1F10/IL-38 (Met1-Trp152)
Accession # AAK68048.1 |
His |
| N-term |
C-term |
|
| Purity |
> 95% as determined by BisTris PAGE
> 95% as determined by HPLC |
| Endotoxin Level |
Less than 1EU per μg by the LAL method. |
| Biological Activity |
Human IL1 Rrp2, hFc Tag captured on CM5 Chip via Protein A can bind IL-1F10/IL-38, His, Human in SPR assay (Biacore T200). Test result was comparable to standard batch. |
| Expression System |
Yeast |
| Theoretical Molecular Weight |
18.07 kDa |
| Apparent Molecular Weight |
Due to glycosylation, the protein migrates to 20-23 kDa based on Bis-Tris PAGE result. |
| Formulation |
Lyophilized from 0.22 μm filtered solution in PBS (pH 7.4). |
| Reconstitution |
Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water. |
| Storage & Stability |
Upon receiving, the product remains stable up to 6 months at -20 °C or below. Upon reconstitution, the product should be stable for 3 months at -80 °C. Avoid repeated freeze-thaw cycles. |
| Target Background |
Interleukin (IL)-38, a newly discovered IL-1 family cytokine, is expressed in several tissues and secreted by various cells. IL-38 has recently been reported to exert an anti-inflammatory function by binding to several receptors, including interleukin-36 receptor (IL-36R), interleukin-1 receptor accessory protein-like 1 (IL-1RAPL1), and interleukin-1 receptor 1 (IL-1R1) to block binding with other pro-inflammatory cytokines and inhibit subsequent signaling pathways; thereby regulating the differentiation and function of T cells, peripheral blood mononuclear cells, macrophages, and dendritic cells. |
| Synonyms |
FIL1 theta; IL-1HY2; IL-1 theta; IL38; |
For research use only. Not intended for human or animal clinical trials, therapeutic or diagnostic use.
