| Species |
Human |
| Protein Construction |
Integrin alpha M beta 2 (ITGAM&ITGB2) Heterodimer [Phe17-Asn1104 (ITGAM) acidic tail and Gln23-Asn700(ITGB2) basic tail]
Accession # P11215-1(ITGAM)&P05107-1(ITGB2) |
His |
| N-term |
C-term |
|
| Purity |
> 95% as determined by Bis-Tris PAGE |
| Endotoxin Level |
Less than 1 EU per μg by the LAL method. |
| Biological Activity |
Measured by its binding ability in a functional ELISA. Immobilized Integrin alpha M beta 2 (ITGAM&ITGB2) Heterodimer, His, Human at 2μg/ml (100 μl/well) on the plate can bind AntiITGB2 Antibody, hFc Tag. Test result was comparable to standard batch. |
| Expression System |
HEK293 |
| Theoretical Molecular Weight |
126.10 kDa (ITGAM) and 79.50 kDa (ITGB2) |
| Apparent Molecular Weight |
Due to glycosylation, the protein migrates to 140-160 kDa (ITGAM) and 90-100 kDa (ITGB2) based on Bis-Tris PAGE result. |
| Formulation |
Lyophilized from 0.22 μm filtered solution in PBS (pH 7.4). |
| Reconstitution |
Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water. |
| Storage & Stability |
Upon receiving, the product remains stable up to 6 months at -20 °C or below. Upon reconstitution, the product should be stable for 3 months at -80 °C. Avoid repeated freeze-thaw cycles. |
| Target Background |
Integrin αMβ2, also called Mac-1 or complement receptor-3 (CR3), is a heterodimer expressed on monocytes. It functions as both an adhesion molecule mediating the diapedesis of leukocytes across the endothelium and a receptor for the iC3b fragment of complement responsible for phagocytic/degranulation responses to microorganisms[1]. Active αMβ2 binds iC3b, coagulation proteins fibrinogen, plasminogen and factor X, extracellular matrix (ECM) proteins fibronectin, laminin and collagen, and cell surface ICAMs, myelin basic protein and DCSIGN[2]. |
| Synonyms |
αMβ2; ITGAM&ITGB2; ITGAM; ITGB2 |
For research use only. Not intended for human or animal clinical trials, therapeutic or diagnostic use.
