| Species |
Human |
| Protein Construction |
Integrin alpha V beta 6 (ITGAV&ITGB6) Heterodimer [Phe31-Val992(ITGAV) acidic tail & Gly22-Asn707(ITGB6) basic tail]
Accession # P06756-1(ITGAV)&P18564-1(ITGB6) |
His |
Avi |
| N-term |
|
C-term |
|
| Purity |
> 95% as determined by BisTris PAGE
> 95% as determined by HPLC |
| Endotoxin Level |
Less than 1 EU per μg by the LAL method. |
| Biological Activity |
Measured by its binding ability in a functional ELISA. Immobilized Integrin alpha V beta 6 (ITGAV&ITGB6) Heterodimer, His & Avi, Human at 2 μg/ml (100μl/well) on the plate can bind Biotinylated Human Latent TGF beta 1, His Tag. Test result was comparable to standard batch. |
| Expression System |
HEK293 |
| Theoretical Molecular Weight |
114 kDa (ITGAV)&79.1 kDa (ITGB6) |
| Apparent Molecular Weight |
Due to glycosylation, the protein migrates to 90-150 kDa based on Bis-Tris PAGE result. |
| Formulation |
Lyophilized from 0.22 μm filtered solution in PBS (pH 7.4). |
| Reconstitution |
Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water. |
| Storage & Stability |
Upon receiving, the product remains stable up to 6 months at -20 °C or below. Upon reconstitution, the product should be stable for 3 months at -80 °C. Avoid repeated freeze-thaw cycles. |
| Target Background |
ITGAV&ITGB6 is a receptor for fibronectin and cytotactin. It recognizes the sequence R-G-D in its ligands. Internalisation of integrin alpha-V/beta-6 via clathrin-mediated endocytosis promotes carcinoma cell invasion. ITGAV&ITGB6 acts as a receptor for fibrillin-1 (FBN1) and mediates R-G-D-dependent cell adhesion to FBN1. |
| Synonyms |
αVβ6; Integrin alpha V beta 6; ITGAV&ITGB6;Integrin alpha V β 6 |
For research use only. Not intended for human or animal clinical trials, therapeutic or diagnostic use.
