| Species |
Human |
| Protein Construction |
ITCH (Arg485-Glu862)_x000D_
Accession # NP_113671.3 |
|
| Purity |
> 95% as determined by BisTris PAGE
> 90% as determined by HPLC |
| Endotoxin Level |
Less than 1EU per μg by the LAL method. |
| Expression System |
E.coli |
| Theoretical Molecular Weight |
44.98 kDa |
| Apparent Molecular Weight |
The protein has a predicted MW of 44.98 kDa same as Bis-Tris PAGE result. |
| Formulation |
Lyophilized from 0.22 μm filtered solution in PBS, 200mM L-Arginine (pH 7.4). |
| Reconstitution |
Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water. |
| Storage & Stability |
Upon receiving, the product remains stable up to 6 months at -20 °C or below. Upon reconstitution, the product should be stable for 3 months at -80 °C. Avoid repeated freeze-thaw cycles. |
| Target Background |
Itch or itchy E3 is a member of the homologous to E6-associated protein C-terminus (HECT)-type family of E3 ligases, with the protein-interacting WW-domains for the recruitment of substrate and the HECT domain for the transfer of ubiquitin to the substrate, and its deletion results in an itchy phenotype with constant skin scratching and multi-organ inflammation. |
| Synonyms |
AIP4; NAPP1; AIF4 |
For research use only. Not intended for human or animal clinical trials, therapeutic or diagnostic use.
