| Species |
Human |
| Protein Construction |
| His |
Latent Activin A (Ser21-Ser426)_x000D_
Accession # P08476 |
| N-term |
C-term |
|
| Purity |
> 95% as determined by Bis-Tris PAGE |
| Endotoxin Level |
Less than 1EU per μg by the LAL method. |
| Biological Activity |
Measured by its ability to inhibit proliferation of MPC11 cells. The ED50 for this effect is 3040ng/mL. Test result was comparable to standard batch. |
| Expression System |
HEK293 |
| Theoretical Molecular Weight |
13.0 kDa (mature form) and 32.0 kDa (pro form) |
| Apparent Molecular Weight |
Due to glycosylation and interchain disulfide bond, the protein migrates to 15 kDa (mature form) and 40-50 kDa (pro form) under reduced (R) condition based on Bis-Tris PAGE result. |
| Formulation |
Lyophilized from 0.22 μm filtered solution in PBS (pH 7.4). |
| Reconstitution |
Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water. |
| Storage & Stability |
Upon receiving, the product remains stable up to 6 months at -20 °C or below. Upon reconstitution, the product should be stable for 3 months at -80 °C. Avoid repeated freeze-thaw cycles. |
| Target Background |
Activin-A, a member of the TGF-β superfamily, Extensive research over the past decades illuminated fundamental roles for activin-A in essential biologic processes, including embryonic development, stem cell maintenance and differentiation, haematopoiesis, cell proliferation and tissue fibrosis. Activin-A signals through two type I and two type II receptors which, upon ligand binding, activate their kinase activity, phosphorylate the SMAD2 and 3 intracellular signaling mediators that form a complex with SMAD4, translocate to the nucleus and activate or silence gene expression. |
| Synonyms |
INHBA; Activin A; INHBA; Activin A; Inhibin beta A chain |
For research use only. Not intended for human or animal clinical trials, therapeutic or diagnostic use.
