| Species |
Human |
| Protein Construction |
| Flag |
His |
LIGHT/TNFSF14 Trimer (Ser89-Val240 Trimer)_x000D_
Accession # O43557-1 |
| N-term |
|
C-term |
|
| Purity |
> 90% as determined by BisTris PAGE
> 95% as determined by HPLC |
| Endotoxin Level |
Less than 1 EU per μg by the LAL method. |
| Biological Activity |
Measured by its binding ability in a functional ELISA. Immobilized LIGHT/TNFSF14 Trimer, His & Flag, Human at 5μg/ml (100 μl/well) on the plate can bind Human HVEM, hFc Tag. Test result was comparable to standard batch. |
| Expression System |
HEK293 |
| Theoretical Molecular Weight |
52.4 kDa |
| Apparent Molecular Weight |
Due to glycosylation, the protein migrates to 53-140 kDa under Non reducing (N) condition based on Bis-Tris PAGE result. |
| Formulation |
Lyophilized from 0.22 μm filtered solution in PBS (pH 7.4). |
| Reconstitution |
Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water. |
| Storage & Stability |
Upon receiving, the product remains stable up to 6 months at -20 °C or below. Upon reconstitution, the product should be stable for 3 months at -80 °C. Avoid repeated freeze-thaw cycles. |
| Target Background |
LIGHT, also known as tumor necrosis factor superfamily member 14 (TNFSF14), is a secreted protein of the TNF superfamily. It is recognized by herpesvirus entry mediator (HVEM), as well as decoy receptor 3. This protein has been shown to stimulate the proliferation of T cells, and trigger apoptosis of various tumor cells. |
| Synonyms |
CD258; TNFSF14; HVEM-L; LIGHT; LTg; TR2 |
For research use only. Not intended for human or animal clinical trials, therapeutic or diagnostic use.
