| Species |
Human |
| Protein Construction |
MASP2 (Gly288-Phe686)_x000D_
Accession # O00187-1 |
His |
| N-term |
C-term |
|
| Purity |
> 95% as determined by BisTris PAGE
> 95% as determined by HPLC |
| Endotoxin Level |
Less than 1 EU per μg by the LAL method. |
| Biological Activity |
Measured by its binding ability in a functional ELISA. Immobilized MASP2, His, Human at 0.5μg/ml (100μl/Well) on the plate can bind AntiMASP2 Antibody, hFc Tag. Test result was comparable to standard batch. |
| Expression System |
E.coli |
| Theoretical Molecular Weight |
46.9 kDa |
| Apparent Molecular Weight |
Due to autocatalytic cleavage, the protein migrates to 18 kDa&25-30 kDa based on Bis-Tris PAGE result. |
| Formulation |
Lyophilized from 0.22 μm filtered solution in 20 mM Tris, 200 mM NaCl (pH 9.0). |
| Reconstitution |
Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water. |
| Storage & Stability |
Upon receiving, the product remains stable up to 6 months at -20 °C or below. Upon reconstitution, the product should be stable for 3 months at -80 °C. Avoid repeated freeze-thaw cycles. |
| Target Background |
The pathogenesis of severe acute respiratory disease syndrome (SARS) is not fully understood. One case-control study has reported an association between susceptibility to SARS and mannan-binding lectin (MBL) in China. As the downstream protein of MBL, variants of the MBL-associated serine protease-2 (MASP2) gene may be associated with SARS coronavirus (SARS-CoV) infection in the same population. |
| Synonyms |
MAP19; MASP-2; MASP1P1; sMap |
For research use only. Not intended for human or animal clinical trials, therapeutic or diagnostic use.
