| Species |
Human |
| Protein Construction |
MASP3 (Ile450-Val721)_x000D_
Accession # P48740-2 |
His |
| N-term |
C-term |
|
| Purity |
> 95% as determined by BisTris PAGE
> 95% as determined by HPLC |
| Endotoxin Level |
Less than 1EU per μg by the LAL method. |
| Biological Activity |
Measured by its ability to cleave a colorimetric peptide substrate, NcarbobenzyloxyLysThioBenzyl ester (ZLysSBzl), in the presence of 5,5'Dithiobis (2nitrobenzoic acid) (DTNB). The specific activity is > 10000 pmol/min/µg. Test result meets the standard. |
| Expression System |
HEK293 |
| Theoretical Molecular Weight |
31.10 kDa |
| Apparent Molecular Weight |
Due to glycosylation, the protein migrates to 43-53 kDa based on Bis-Tris PAGE result. |
| Formulation |
Lyophilized from 0.22 μm filtered solution in PBS (pH 7.4). |
| Reconstitution |
Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water. |
| Storage & Stability |
Upon receiving, the product remains stable up to 6 months at -20 °C or below. Upon reconstitution, the product should be stable for 3 months at -80 °C. Avoid repeated freeze-thaw cycles. |
| Target Background |
Mannose-binding lectin-associated serine protease-1 (also named MASP3), a multifunctional serine protease, plays an important role in innate immunity which is capable of activating the lectin pathway of the complement system and also triggering coagulation cascade system. |
| Synonyms |
MASP-3; MASP-1; MASP1; RaRF; Serine protease 5; CRARF; CRARF1; PRSS5; |
For research use only. Not intended for human or animal clinical trials, therapeutic or diagnostic use.
