| Species |
Human |
| Protein Construction |
Mature TGF beta 2 (Ala303-Ser414)_x000D_
Accession # P61812-1 |
|
| Purity |
> 95% as determined by Bis-Tris PAGE |
| Endotoxin Level |
Less than 1EU per μg by the LAL method. |
| Biological Activity |
Measured by its binding ability in a functional ELISA. Immobilized Mature TGF beta 2, Human at 5μg/ml (100μl/Well) on the plate can bind Biotinylated Human TGFbeta RII, mFc Tag. Test result was comparable to standard batch.
Measured by its ability to inhibit the IL4dependent proliferation of TF1 cells. The ED50 for this effect is 0.1 1 ng/ml. Test result was comparable to standard batch. |
| Expression System |
HEK293 |
| Theoretical Molecular Weight |
12.7 kDa |
| Apparent Molecular Weight |
Due to glycosylation, the protein migrates to 13-15 kDa based on Bis-Tris PAGE result. |
| Formulation |
Lyophilized from 0.22μm filtered solution in 4mM HCl. |
| Reconstitution |
Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in 4mM HCl. |
| Storage & Stability |
Upon receiving, the product remains stable up to 6 months at -20 °C or below. Upon reconstitution, the product should be stable for 3 months at -80 °C. Avoid repeated freeze-thaw cycles. |
| Target Background |
Transforming growth factor beta(2) (TGF-beta(2)), a growth regulator of human lens epithelial cells (HLECs), also regulates the death of these cells. TGF-beta(2)-induced apoptosis in HLECs was preceded by an induction of reactive oxygen species (ROS) and a decrease in glutathione in the intracellular content, indicating that this factor induces oxidative stress in HLECs. |
| Synonyms |
TGFB2; TGFBR2; TGFR2; TbetaR-II; TGFβR2; BB105277; TGF-beta 2; TβR-II; TGF-β 2 |
For research use only. Not intended for human or animal clinical trials, therapeutic or diagnostic use.
