| Species |
Human |
| Protein Construction |
M-CSF/CSF-1 (Glu33-Asn190)_x000D_
Accession # P09603-1 |
|
| Purity |
> 95% as determined by BisTris PAGE
> 95% as determined by HPLC |
| Endotoxin Level |
Less than 0.05EU per μg by the LAL method. |
| Biological Activity |
Measured by its binding ability in a functional ELISA. Immobilized M-CSF/CSF-1, Human at 0.5μg/ml (100μl/Well) on the plate can bind Human MCSF R, hFc Tag. Test result was comparable to standard batch.
Measured in a cell proliferation assay using RAW264.7 cells. The ED50 for this effect is 2.5 7.0 ng/mL. Test result was comparable to standard batch. |
| Expression System |
HEK293 |
| Theoretical Molecular Weight |
18.40 kDa |
| Apparent Molecular Weight |
Due to glycosylation, the protein migrates to 23-28 kDa based on Bis-Tris PAGE result. |
| Formulation |
Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). |
| Reconstitution |
Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water. |
| Storage & Stability |
Upon receiving, the product remains stable up to 6 months at -20 °C or below. Upon reconstitution, the product should be stable for 3 months at -80 °C. Avoid repeated freeze-thaw cycles. |
| Target Background |
CSF-1, also known as M-CSF , is a four alpha -helical-bundle cytokine that is the primary regulator of macrophage survival, proliferation and differentiation. CSF-1 is also essential for the survival and proliferation of osteoclast progenitors.CSF-1 is cytokine that plays an essential role in the regulation of survival, proliferation and differentiation of hematopoietic precursor cells, especially mononuclear phagocytes, such as macrophages and monocytes. Promotes the release of proinflammatory chemokines, and thereby plays an important role in innate immunity and in inflammatory processes. This mutation leads to a deletion of exons 2-7 of the EGFR gene and renders the mutant receptor incapable of binding any known ligand. |
| Synonyms |
CSF1; CSF-1; MCSF; M-CSF; MGC31930; Lanimostim |
For research use only. Not intended for human or animal clinical trials, therapeutic or diagnostic use.
